Abstract

We describe the structural characterization of the capsular polysaccharides (CPSs) of Pasteurella multocida serotypes B and E. CPS was isolated following organic solvent precipitation of the supernatant from flask grown cells. Structural analysis utilizing nuclear magnetic resonance spectroscopy enabled the determination of the CPS structures and revealed significant structural similarities between the two serotypes, but also provided an explanation for the serological distinction. This observation was extended by the development of polyclonal sera to the glycoconjugate of serotype B CPS that corroborated the structural likenesses and differences. Finally, identification of these structures enabled a more comprehensive interrogation of the genetic loci and prediction of roles for some of the encoded proteins in repeat unit biosynthesis.

中文翻译：

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多杀性巴氏杆菌 B 和 E 血清型的荚膜多糖：结构、遗传和血清学比较

摘要

我们描述了多杀性巴氏杆菌荚膜多糖 (CPS) 的结构特征血清型 B 和 E。在有机溶剂沉淀培养瓶生长细胞的上清液后分离 CPS。利用核磁共振光谱的结构分析能够确定 CPS 结构并揭示两种血清型之间的显着结构相似性，但也为血清学区别提供了解释。通过将多克隆血清发展成血清型 B CPS 的糖缀合物，证实了结构相似性和差异，这一观察得到了扩展。最后，对这些结构的鉴定能够更全面地研究遗传基因座并预测一些编码蛋白质在重复单元生物合成中的作用。