BACKGROUND The increasing and inappropriate use of antibiotics has increased the number of multidrug-resistant microorganisms to these drugs, causing the emergence of infections that are difficult to control and manage by health professionals. As an alternative to combat these pathogens, some monoterpenes have harmful effects on the bacterial cell membrane, showing themselves as an alternative in combating microorganisms. Therefore, the positive enantiomer α -pinene becomes an alternative to fight bacteria, since it was able to inhibit the growth of the species Escherichia coli ATCC 25922, demonstrating the possibility of its use as an isolated antimicrobial or associated with other drugs. AIMS The aim of this study is to evaluate the sensitivity profile of E. coli ATCC 25922 strain against clinical antimicrobials associated with (+) -α-pinene and how it behaves after successive exposures to subinhibitory concentrations of the phytochemicals. METHODS The minimum inhibitory concentration (MIC) was determined using the microdilution method. The study of the modulating effect of (+) -α-pinene on the activity of antibiotics for clinical use in strains of E. coli and the analysis of the strain's adaptation to the monoterpene were tested using the adapted disk-diffusion method. RESULTS The results demonstrate that the association of monoterpene with the antimicrobials ceftazidime, amoxicillin, cefepime, cefoxitin and amikacin is positive since it leads to the potentiation of the antibiotic effect of these compounds. It was observed that the monoterpene was able to induce crossresistance only for antimicrobials: cefuroxime, ceftazidime, cefepime and chloramphenicol. CONCLUSION It is necessary to obtain more concrete data for the safe use of these combinations, paying attention to the existence of some type of existing toxicity reaction related to the herbal medicine and to understand the resistance mechanisms acquired by the microorganism.

中文翻译：

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(+)-α-蒎烯与商业抗菌剂对大肠杆菌菌株适应性的关联和评价的影响。

背景抗生素使用的增加和不当使用增加了对这些药物的多重耐药微生物的数量，导致出现难以由卫生专业人员控制和管理的感染。作为对抗这些病原体的替代方法，一些单萜对细菌细胞膜有有害影响，表明它们是对抗微生物的替代方法。因此，正对映异构体 α-蒎烯成为对抗细菌的替代品，因为它能够抑制大肠杆菌 ATCC 25922 物种的生长，证明其用作分离的抗菌剂或与其他药物相关的可能性。目的 本研究的目的是评估大肠杆菌的敏感性特征。大肠杆菌 ATCC 25922 菌株对抗与 (+) -α-蒎烯相关的临床抗菌剂及其在连续暴露于亚抑制浓度的植物化学物质后的表现。方法采用微量稀释法测定最低抑菌浓度（MIC）。(+)-α-蒎烯对大肠杆菌菌株临床用抗生素活性的调节作用研究及菌株对单萜的适应性分析采用适应性圆盘扩散法进行测试。结果 结果表明，单萜与抗微生物剂头孢他啶、阿莫西林、头孢吡肟、头孢西丁和阿米卡星的关联是积极的，因为它导致这些化合物的抗生素作用增强。观察到单萜能够仅对抗菌剂产生交叉耐药性：头孢呋辛、头孢他啶、头孢吡肟和氯霉素。结论有必要为这些组合的安全使用获得更具体的数据，注意与草药相关的某些类型的现有毒性反应的存在，并了解微生物获得的抗性机制。