Here, we describe a novel mycovirus, Auricularia heimuer fusarivirus 1 (AhFV1), isolated from the edible fungus Auricularia heimuer strain CCMJ1296. The virus has a single-stranded positive-sense [+ssRNA] genome of 7,127 nucleotides containing two overlapping open reading frames (ORFs) and a poly(A) tail. The large ORF1 encodes a polyprotein of 1,637 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and DEAD-like helicase superfamily (DEXDc) domains. ORF2 encodes a putative 633-aa protein with unknown function. A BLAST search showed that the nucleotide sequence of the AhFV1 genome is 41.28% identical to that of Sclerotium rolfsii fusarivirus 2 and 40.49% identical to that of Sclerotium rolfsii fusarivirus 1. Phylogenetic analysis based on RdRp and helicase (Hel) sequences indicated that AhFV1 is related to unclassified mycoviruses and other fusariviruses. Our data suggest that AhFV1 should be classified as a member of the newly proposed family “Fusariviridae”. This is the second virus and the first full genome sequence of a fusarivirus from A. heimuer.

在这里，我们描述了一种新型的分枝杆菌病毒，从食用菌Auicularia heimuer中分离出的海默乌耳镰刀状病毒1（AhFV1）菌株CCMJ1296。该病毒具有7,127个核苷酸的单链正[+ ssRNA]基因组，其中包含两个重叠的开放阅读框（ORF）和一个poly（A）尾巴。大的ORF1编码具有保守的RNA依赖性RNA聚合酶（RdRp）和DEAD样解旋酶超家族（DEXDc）域的1637个氨基酸（aa）的多蛋白。ORF2编码功能未知的633-aa蛋白。BLAST搜索显示，AhFV1基因组的核苷酸序列与罗氏菌伏尔氏菌2的核苷酸序列具有41.28％的同一性，与罗氏菌伏尔氏菌1具有40.49％的序列相同性。与未分类的分枝杆菌病毒和其他融合病毒有关。我们的数据表明，AhFV1应该归类为新提议的家族的成员“Fusariviridae ”。这是来自heimuer的融合病毒的第二个病毒和第一个全基因组序列。