Fungal enzymes are widely used in technological processes and have some interesting features to be applied in a variety of biosynthetic courses. Here, free and substrate-immobilised lipases from Fusarium verticillioides P24 were obtained by solid-state fermentation using wheat bran as substrate and fungal carrier. Based on their hydrolytic and transesterification activities, the lipases were characterised as pH-dependent in both reactions, with higher substrate conversion in an alkaline environment. Thermally, the lipases performed well from 30 to 45 °C, being more stable in mild conditions. Organic solvents significantly influenced the lipase selectivity using different vegetable oils as fatty acid source. Omega(ω)-3 production in n-hexane achieved 45% using canola oil, against ≈ 18% in cyclohexane. However, ω-6 production was preferably produced for both solvents using linseed oil with significant alterations in the yield (≈ 79% and 49% for n-hexane and cyclohexane, respectively). Moreover, the greatest enzyme selectivity for ω-6 led us to suppose a lipase preference for the Sn1 position of the triacylglycerol. Lastly, a transesterification reaction was performed, achieving 90% of ester conversion in 72 h. This study reports the characterisation and use of free and substrate-immobilised lipases from Fusarium verticillioides P24 as an economic and efficient method for the first time.

真菌酶广泛用于技术过程中，并具有一些有趣的功能，可应用于各种生物合成过程。在这里，利用小麦麸皮作为底物和真菌载体，通过固态发酵获得了镰刀镰刀菌P24的游离的和固定化底物的脂肪酶。基于它们的水解和酯交换活性，脂肪酶在两个反应中均具有pH依赖性，在碱性环境中具有较高的底物转化率。在热方面，脂肪酶在30至45°C的温度下表现良好，在温和条件下更稳定。使用不同的植物油作为脂肪酸源，有机溶剂会显着影响脂肪酶的选择性。欧米加（ω）-3生产Ñ使用低芥酸菜子油的正己烷含量达到45％，而环己烷中的含量约为18％。然而，ω-6的产生是优选产生用于使用亚麻子油与（≈79％和49％屈服显著改变两种溶剂Ñ分别正己烷和环己烷）。此外，对ω-6的最大酶选择性导致我们假设脂肪酶对三酰基甘油的Sn1位置具有偏好。最后，进行酯交换反应，在72小时内达到90％的酯转化率。这项研究首次报道了镰刀镰刀菌（Pusarium v​​erticillioides） P24中游离的和固定化底物的脂肪酶的表征和使用，这是首次将其作为经济有效的方法。