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Enhanced Production of Astaxanthin without Decrease of DHA Content in Aurantiochytrium limacinum by Overexpressing Multifunctional Carotenoid Synthase Gene.
Applied Biochemistry and Biotechnology ( IF 3 ) Pub Date : 2020-08-18 , DOI: 10.1007/s12010-020-03403-w
Yuki Kubo 1 , Mai Shiroi 1 , Tokuhiro Higashine 1 , Yuki Mori 1 , Daichi Morimoto 1 , Satoshi Nakagawa 1 , Shigeki Sawayama 1
Affiliation  

Aurantiochytrium limacinum produces both docosahexaenoic acid (DHA) and astaxanthin, respectively. Organisms that produce these industrially important materials more efficiently than microalgae are currently needed. In this study, we overexpressed a putative homolog of CarS, which is involved in synthesizing the astaxanthin precursor, β-carotene, in A. limacinum to increase carotenoid synthesis with the goal of obtaining strains that produce large amounts of both DHA and carotenoids. AlCarS transformants #1 and #18 produced significantly increased amounts of astaxanthin as assessed according to culture (up to 5.8-fold) and optical density (up to 9.3-fold). The improved astaxanthin production of these strains did not affect their DHA productivity. Additionally, their CarS expression levels were higher than those of the wild-type strain, suggesting that CarS overexpression enhanced β-carotene production, which in turn improved astaxanthin productivity. Although cell yields were slightly decreased, these features will be valuable in health food, medical care, and animal feed fields.

中文翻译:

通过过量表达多功能类胡萝卜素合酶基因,增强虾青素的生产,而又不降低桔红金藻中DHA含量。

金黄色葡萄球菌分别产生二十二碳六烯酸(DHA)和虾青素。当前需要比微藻更有效地生产这些工业上重要的材料的生物。在这项研究中,我们过表达CarS的假定同源物,该同源物参与了在A. limacinum中合成虾青素前体β-胡萝卜素,以增加类胡萝卜素的合成,目的是获得可产生大量DHA和类胡萝卜素的菌株。根据培养物(最多5.8倍)和光密度(最多9.3倍)评估,AlCarS转化体#1和#18产生的虾青素含量显着增加。这些菌株虾青素产量的提高并不影响其DHA生产率。此外,它们的CarS表达水平高于野生型菌株,提示CarS过表达增强了β-胡萝卜素的产生,从而提高了虾青素的生产率。尽管细胞产量略有下降,但这些功能在保健食品,医疗保健和动物饲料领域将是有价值的。
更新日期:2020-08-18
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