To evaluate the antimicrobial and microbicidel activity of B. radicata fermentation broth, the broth was purified by DEAE-cellulose and sephadex LC-20 column. The compounds were submitted to spectral analyses (HPLC, FT-IR, 1D and 2D NMR etc.). The purified compounds were identified as the Griseococcin(s) which were naphthoquinone derivatives, the Chemical formula and MW of Griseococcin (1) was determined as C37O10H43N and 661 Da. only Griseococcin (1) has good antimicrobial activity among the Griseococcin(s). The zone of inhibition (ZOI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC) of Griseococcin (1) were used to investigate the antimicrobial activity. Antifungal activity of Griseococcin (1) was significant, especially for main pathogenic fungus Trichophyton rubrum and Trichophyton mentagrophytes, MFC/MIC of Griseococcin (1) was 1, while MFC/MIC of postive control was greater than 4, the fungicidal effect of Griseococcin (1) was better than that of positive control. In this paper, the secondary metabolite compound Griseococcin (1) from B. radicata was purified. The purified compound can restrain main pathogens (T. rubrum and T. mentagrophytes) leading to tinea pedis. The antifungal activity of Griseococcin (1) was similar to that of the positive control and the fungicidal effect of Griseococcin (1) was better than that of positive control, it might be suitable for pharmaceutical industries.

中文翻译：

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来自Bovistella radicata (Mont.) Pat 的灰球菌素 (1) 和抗真菌活性。

为了评估 B. radicata 发酵液的抗菌和杀微生物活性，该发酵液通过 DEAE-纤维素和 sephadex LC-20 柱进行纯化。对化合物进行光谱分析（HPLC、FT-IR、1D 和 2D NMR 等）。纯化的化合物被鉴定为灰球菌素，它是萘醌衍生物，灰球菌素 (1) 的化学式和分子量确定为 C37O10H43N 和 661 Da。在灰球菌素中，只有灰球菌素 (1) 具有良好的抗菌活性。灰球菌素 (1) 的抑菌圈 (ZOI)、最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC) 或最低杀菌浓度 (MFC) 用于研究抗菌活性。灰球菌素 (1) 的抗真菌活性显着，特别是对于主要病原真菌红色毛癣菌和须癣毛癣菌，灰球菌素(1)的MFC/MIC为1，而阳性对照的MFC/MIC大于4，灰球菌素(1)的杀菌效果优于阳性对照。在本文中，从B. radicata 中纯化了次级代谢产物Griseococcin (1)。纯化的化合物可以抑制导致足癣的主要病原体（红色毛癣菌和须癣毛癣菌）。灰球菌素(1)的抗真菌活性与阳性对照相似，且灰球菌素(1)的杀菌效果优于阳性对照，可能适用于制药行业。灰球菌素(1)的杀菌效果优于阳性对照。在本文中，从 B. radicata 中纯化了次级代谢物化合物灰球菌素 (1)。纯化的化合物可以抑制导致足癣的主要病原体（红色毛癣菌和须癣毛癣菌）。灰球菌素(1)的抗真菌活性与阳性对照相似，且灰球菌素(1)的杀菌效果优于阳性对照，可能适用于制药行业。灰球菌素(1)的杀菌效果优于阳性对照。在本文中，从B. radicata 中纯化了次级代谢产物Griseococcin (1)。纯化的化合物可以抑制导致足癣的主要病原体（红色毛癣菌和须癣毛癣菌）。灰球菌素(1)的抗真菌活性与阳性对照相似，且灰球菌素(1)的杀菌效果优于阳性对照，可能适用于制药行业。