Endostemon tereticaulis (poir.) M.Ashby is a species of the Lamiaceae family present in Niger. This plant is used in traditional medicine due to its various biological potentialities. The present study investigated the chemical composition of the essential oil and the antibacterial activity of the essential oil and ethanolic extract of Endostemon tereticaulis against resistant pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the essential oil led to the identification of 43 compounds representing 99.55% of the total essential oil. The major components were caryophyllene oxide (15.17%) followed by α-humulene (13.96%), α-copaene (11.75%), (E)-β-caryophyllene (8.44%), and δ-cadinene (6.78%). The antibacterial activity was tested against multiresistant Acinetobacter baumannii P1483, Salmonella spp. H1548, extended-spectrum β-lactamase-Escherichia coli Bu8566, Enterobacter cloacae Bu147, Proteus mirabilis Bu190, Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Enterococcus faecium H3434, methicillin-resistant Staphylococcus aureus P1123, and Staphylococcus aureus (ATCC 25923). The antibacterial assays revealed that the essential oil was more active than the ethanolic extract against the studied bacteria with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values ranging from 0.06 to 2 mg/mL. Also, the ethanolic extract was effective against the bacteria tested with MIC and MBC values ranging from 0.12 to 3 mg/mL. This study showed that Endostemon tereticaulis essential oil is rich in bioactive compounds. Ethanolic extract and essential oil exhibited potential antibacterial activity. These results provide a scientific basis for the use of this plant in traditional medicine. The current study described for the first time the antibacterial activity of Endostemon tereticaulis.

内生造口虫（po）M.Ashby是尼日尔的唇形科的一种。由于具有多种生物潜力，该植物可用于传统医学。本实验研究的精油和精油和的乙醇提取物的抗菌活性的化学组成Endostemon tereticaulis对耐致病菌。气相色谱-质谱联用分析精油可鉴定出43种化合物，占精油总量的99.55％。主要成分是石竹烯氧化物（15.17％），其次是α-腐殖烯（13.96％），α-古丁烯（11.75％），（E）-β-石竹烯（8.44％）和δ--烯（6.78％）。测试了抗多药耐药性鲍曼不动杆菌P1483，沙门氏菌属。H1548，广谱β内酰胺酶大肠杆菌Bu8566，阴沟肠杆菌Bu147，奇异变形杆菌Bu190 ，铜绿假单胞菌（ATCC 27853），肺炎克雷伯氏菌（ATCC 700603），大肠杆菌（ATCC 25922），屎肠球菌H3434，耐甲氧西林金黄色葡萄球菌金黄色葡萄球菌P1123和金黄色葡萄球菌（ATCC 25923）。抗菌测定表明，精油比乙醇提取物对所研究细菌的活性更高，最小抑菌浓度（MIC）和最小杀菌浓度（MBC）值在0.06至2 mg / mL范围内。而且，乙醇提取物对MIC和MBC值在0.12至3 mg / mL范围内的细菌均有效。这项研究表明，Endostemon tereticaulis精油富含生物活性化合物。乙醇提取物和精油表现出潜在的抗菌活性。这些结果为该植物在传统医学中的使用提供了科学依据。目前的研究首次描述了内生梯形内皮菌（Endostemon tereticaulis）的抗菌活性。