The organic compound di(2-ethylhexyl) phthalate (DEHP) is widely used as a plasticizer in many products. Exposure to DEHP has been reported to lead to adverse pregnancy outcomes by suppressing placenta growth and development. The aim of this study was to determine the gene expression profiles of rat placenta exposed to (DEHP) and identify genes crucial for the DEHP response. Three groups of Wistar rats were administered an intragastric dose of 1,000 mg/kg DEHP, 500 mg/kg DEHP, or corn oil, RNA was isolated from placenta tissue, and hybridization was performed. Gene expression profiles were analyzed by identifying functional enrichment, differentially expressed genes (DEGs), protein–protein interaction (PPI) networks and modules, and transcription factor (TF)-miRNA-target regulatory networks. We obtained 2,032 DEGs, including cytochrome P450, family 2, subfamily R, polypeptide 1 (CYP2R1), sterol O-acyltransferase 2 (SOAT2), and 24-dehydrocholesterol reductase (DHCR24) from the steroid biosynthesis pathway and somatostatin receptor 4 (SSTR4) and somatostatin receptor 2 (SSTR2) in the neuroactive ligand-receptor interaction pathway. The PPI network included 476 nodes, 2,682 interaction pairs, and three sub-network modules. Moreover, eight miRNAs, three TFs, and 176 regulatory pairs were obtained from the TF-miRNA-target regulatory network. CYP2R1, SOAT2, DHCR24, SSTR4, and SSTR2 may affect DEHP influence on rat placenta development.

有机化合物邻苯二甲酸二（2-乙基己基）酯（DEHP）被广泛用作许多产品中的增塑剂。据报道，接触 DEHP 会抑制胎盘的生长和发育，从而导致不良的妊娠结局。本研究的目的是确定暴露于 (DEHP) 的大鼠胎盘的基因表达谱，并确定对 DEHP 反应至关重要的基因。三组Wistar大鼠灌胃1,000 mg/kg DEHP、500 mg/kg DEHP或玉米油，从胎盘组织中分离RNA，进行杂交。通过识别功能富集、差异表达基因 (DEG)、蛋白质-蛋白质相互作用 (PPI) 网络和模块以及转录因子 (TF)-miRNA-靶标调控网络来分析基因表达谱。我们获得了 2,032 个 DEG，包括细胞色素 P450、家族 2、亚家族 R、多肽 1 (CYP2R1)、甾醇 O-酰基转移酶 2 (SOAT2) 和 24-脱氢胆固醇还原酶 (DHCR24) 来自类固醇生物合成途径和生长抑素受体 4 (SSTR4) 和生长抑素受体 2 (SSTR2)神经活性配体-受体相互作用途径。PPI 网络包括 476 个节点、2,682 个交互对和三个子网模块。此外，从 TF-miRNA-靶标调控网络中获得了 8 个 miRNA、3 个 TF 和 176 个调控对。CYP2R1、SOAT2、DHCR24、SSTR4 和 SSTR2 可能会影响 DEHP 对大鼠胎盘发育的影响。和来自类固醇生物合成途径的 24-脱氢胆固醇还原酶 (DHCR24) 和神经活性配体-受体相互作用途径中的生长抑素受体 4 (SSTR4) 和生长抑素受体 2 (SSTR2)。PPI 网络包括 476 个节点、2,682 个交互对和三个子网模块。此外，从 TF-miRNA-靶标调控网络中获得了 8 个 miRNA、3 个 TF 和 176 个调控对。CYP2R1、SOAT2、DHCR24、SSTR4 和 SSTR2 可能会影响 DEHP 对大鼠胎盘发育的影响。和来自类固醇生物合成途径的 24-脱氢胆固醇还原酶 (DHCR24) 和神经活性配体-受体相互作用途径中的生长抑素受体 4 (SSTR4) 和生长抑素受体 2 (SSTR2)。PPI 网络包括 476 个节点、2,682 个交互对和三个子网模块。此外，从 TF-miRNA-靶标调控网络中获得了 8 个 miRNA、3 个 TF 和 176 个调控对。CYP2R1、SOAT2、DHCR24、SSTR4 和 SSTR2 可能会影响 DEHP 对大鼠胎盘发育的影响。