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Hepatoprotective effect of Omega-3 PUFAs against acute paracetamol-induced hepatic injury confirmed by FTIR.
Human & Experimental Toxicology ( IF 2.8 ) Pub Date : 2020-09-10 , DOI: 10.1177/0960327120954522
Zeinab A El-Gendy 1 , Seham A El-Batran 1 , Sah Youssef 2 , A Ramadan 2 , Walid El Hotaby 3 , Rofanda M Bakeer 4, 5 , Rania F Ahmed 1
Affiliation  

Acute paracetamol over dose-induced hepatotoxicity is considered an important medical hazard especially among women. Omega-3 long-chain polyunsaturated fatty acids (Omega-3 PUFAs) daily doses are nowadays recommended for their antioxidant and anti-inflammatory potentials. Fourier transform infrared (FTIR) spectroscopy is considered a reliable method in analyzing cellular alterations and is now efficiently used to diagnose several diseases and the efficacy of drugs even in the early stages. The aim of our study was to evaluate the hepatoprotective effect of Omega-3 PUFAs against paracetamol-induced hepatotoxicity in rats confirmed through measuring protein alterations in hepatocytes by FTIR. Rats were pretreated with Omega-3 PUFAs (50 and 100 mg/kg) for 21 days prior to oral ingestion of paracetamol. FTIR results revealed that Omega-3 PUFAs (50 mg/kg) limited the toxic effects of paracetamol by restoring the hepatic amide I to amide II ratio. In addition; biochemical analyses demonstrated that serum ALT, AST, Cholesterol, LDL-cholesterol and Il-6 levels as well as hepatic TNF-α, MDA, NOx levels were decreased. Besides; serum HDL-cholesterol level and hepatic GSH level were increased. Histopathological examinations of hepatic sections validated the hepatoprotective potential. The overall effect of this dose was comparable to those of the usual recommended hepatoprotective supplement; silymarin. In conclusion; it would be recommended to use Omega-3 PUFAs in low doses on daily bases as a hepatoprotective agent.



中文翻译:

FTIR 证实 Omega-3 PUFA 对急性扑热息痛诱导的肝损伤的保肝作用。

急性扑热息痛超过剂量引起的肝毒性被认为是一种重要的医疗危害,尤其是在女性中。Omega-3 长链多不饱和脂肪酸 (Omega-3 PUFA) 的每日剂量因其抗氧化和抗炎潜力而被推荐使用。傅里叶变换红外 (FTIR) 光谱被认为是分析细胞变化的可靠方法,现在即使在早期阶段也能有效地用于诊断多种疾病和药物的功效。我们研究的目的是评估 Omega-3 多不饱和脂肪酸对扑热息痛诱导的大鼠肝毒性的保肝作用,通过 FTIR 测量肝细胞中的蛋白质变化得到证实。在口服扑热息痛之前,大鼠用 Omega-3 多不饱和脂肪酸(50 和 100 毫克/千克)预处理 21 天。FTIR 结果显示,Omega-3 PUFA (50 mg/kg) 通过恢复肝酰胺 I 与酰胺 II 的比例来限制扑热息痛的毒性作用。此外; 生化分析表明,血清 ALT、AST、胆固醇、低密度脂蛋白胆固醇和 Il-6 水平以及肝脏 TNF-α、MDA、NOx 水平降低。除了; 血清高密度脂蛋白胆固醇水平和肝脏谷胱甘肽水平升高。肝脏切片的组织病理学检查证实了其保肝潜力。该剂量的总体效果与通常推荐的保肝补充剂相当;水飞蓟素。综上所述; 建议每天使用低剂量的 Omega-3 多不饱和脂肪酸作为保肝剂。生化分析表明,血清 ALT、AST、胆固醇、低密度脂蛋白胆固醇和 Il-6 水平以及肝脏 TNF-α、MDA、NOx 水平降低。除了; 血清高密度脂蛋白胆固醇水平和肝脏谷胱甘肽水平升高。肝脏切片的组织病理学检查证实了其保肝潜力。该剂量的总体效果与通常推荐的保肝补充剂相当;水飞蓟素。综上所述; 建议每天使用低剂量的 Omega-3 多不饱和脂肪酸作为保肝剂。生化分析表明,血清 ALT、AST、胆固醇、低密度脂蛋白胆固醇和 Il-6 水平以及肝脏 TNF-α、MDA、NOx 水平降低。除了; 血清高密度脂蛋白胆固醇水平和肝脏谷胱甘肽水平升高。肝脏切片的组织病理学检查证实了其保肝潜力。该剂量的总体效果与通常推荐的保肝补充剂相当;水飞蓟素。综上所述; 建议每天使用低剂量的 Omega-3 多不饱和脂肪酸作为保肝剂。该剂量的总体效果与通常推荐的保肝补充剂相当;水飞蓟素。综上所述; 建议每天使用低剂量的 Omega-3 多不饱和脂肪酸作为保肝剂。该剂量的总体效果与通常推荐的保肝补充剂相当;水飞蓟素。综上所述; 建议每天使用低剂量的 Omega-3 多不饱和脂肪酸作为保肝剂。

更新日期:2020-09-10
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