During April and May 2020, we studied 20 hospitalized COVID-19 patients, their hospital rooms (fomites and aerosols), and their close contacts for molecular and culture evidence of SARS-CoV-2 virus. Among the more than 400 samples, we found molecular evidence of virus in most sample types, especially the nasopharygeal (NP), saliva, and fecal samples, but the prevalence of molecular positivity among fomites and aerosols was low. The agreement between NP swab and saliva positivity was high (89.5%, Kappa 0.79). Two NP swabs collected from patients on one and seven days post-symptom onset had evidence of infectious virus (2 passages over 14 days in Vero E6 cells). In summary, the low molecular prevalence and lack of viable SARS-CoV-2 virus in fomites and air samples implied low nosocomial risk SARS-CoV-2 transmission through inanimate objects or aerosols.

中文翻译：

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住院COVID-19患者中的环境和雾化SARS-CoV-2。

在2020年4月和2020年5月，我们研究了20例住院的COVID-19患者，其病房（烟雾和气雾剂）及其密切接触者的SARS-CoV-2病毒的分子和培养证据。在400多个样本中，我们在大多数样本类型中都发现了病毒的分子证据，尤其是鼻咽（NP），唾液和粪便样本，但是在毒蛇毒和气溶胶中，分子阳性的发生率很低。NP拭子与唾液阳性之间的一致性很高（89.5％，Kappa为0.79）。在症状发作后的第1天和第7天从患者身上收集到的两个NP棉签有感染性病毒的证据（在Vero E6细胞中，在14天中传了2代）。综上所述，