Liquid phase electron microscopy is a new analytical method that has opened up a rapidly emerging field of research during the past decade. This article discusses this new microscopy modality within the context of imaging eukaryotic cells, bacteria, proteins, viruses, and biomineralization processes. The obtained resolution is typically not a function of the instrument, rather it is limited by the available electron dose within the limit of radiation damage. Therefore, different types of samples are best imaged with different electron microscopy (EM) modalities. The obtained information differs from that acquired with conventional EM as well as cryo-electron microscopy. This article gives an overview of achievements thus far in this area and the unique information that has been obtained. A discussion on potential future developments in the field, and technological advancements required to reach those goals conclude the article.

液相电子显微镜是一种新的分析方法，在过去的十年中打开了一个迅速兴起的研究领域。本文在成像真核细胞，细菌，蛋白质，病毒和生物矿化过程的背景下讨论了这种新的显微镜方法。所获得的分辨率通常不是仪器的功能，而是受辐射损伤范围内可用电子剂量的限制。因此，不同类型的样品最好用不同的电子显微镜（EM）模式成像。所获得的信息与常规EM以及冷冻电子显微镜所获得的信息不同。本文概述了迄今为止在该领域取得的成就以及已获得的独特信息。讨论该领域的潜在未来发展，