Collagen fibers and inflammatory cells are the basis for jenny endometrium Kenney and Doig's classification developed for the mare. The infiltration of a large number of eosinophils in the jenny endometrium is intriguing. Eosinophil and fibroblast produced IL33, which has been related to fibrosis development and chronicity. This work on the endometrium consisted of (i) quantification of collagen type I (COL1A2), type III (COL3A1), and IL33 transcripts; (ii) histological localization and quantification of COL1 and COL3 proteins; and (iii) eosinophil and neutrophil count and correlation with collagen area and IL33 transcripts. Localization of COL protein in the jenny endometrium was also compared to the mare endometrium. As fibrosis increased, eosinophil and neutrophil count decreased (P < 0.05). A 5-fold increase in IL33 transcripts was noted from categories IIA to III. There was a tendency toward a positive correlation between eosinophil count and IL33 transcripts in category IIA endometrium (P = 0.055). Neither transcripts of COL1A2 nor COL3A1 nor the areas of COL1 or COL3 differed with endometrial categories. Unlike for the mare, and regardless of the jenny endometrium classification, COL3 was always found to different extents in the stratum compactum, while COL1 was mainly present in deep stroma. As fibrosis progressed in the mare, an extensive increase in COL1 fibers was notorious under the surface epithelium. Correlations between neutrophil count and COL1 and COL3 areas were observed in the jenny endometrium, although no correlation was found for eosinophil count. Neutrophil count positive correlation with the COL1 area and negative correlation with the COL3 area in endometria with mild lesions suggest that neutrophils in the jenny endometrium may be involved in fibrogenesis. In addition, when eosinophilia subsides, the endometrium reacts with fibrosis establishment, which could be stimulated by the pro-fibrotic cytokine IL33, whose release might then be ascribed to fibroblasts. Further studies are needed to analyze the effect of the presence of COL3 next to the surface epithelium in the stratum compactum, or around the endometrial glands on jenny's endometrial function and fertility.

胶原纤维和炎性细胞是詹妮子宫内膜的基础肯尼和杜伊格为母马开发的分类。珍妮子宫内膜中大量嗜酸性粒细胞的浸润令人感兴趣。嗜酸性粒细胞和成纤维细胞产生IL33，这与纤维化的发展和慢性有关。子宫内膜的这项工作包括（i）定量I型胶原（COL1A2），III型胶原（COL3A1）和IL33转录本；（ii）COL1和COL3蛋白的组织学定位和定量；（iii）嗜酸性粒细胞和嗜中性粒细胞计数及其与胶原面积和IL33转录物的相关性。还比较了珍妮子宫内膜和母马子宫内膜中COL蛋白的定位。随着纤维化的增加，嗜酸性粒细胞和中性粒细胞计数减少（P<0.05）。从IIA到III类，IL33转录物增加了5倍。IIA类子宫内膜的嗜酸性粒细胞计数与IL33转录本之间呈正相关趋势（P= 0.055）。子宫内膜类别既无COL1A2，COL3A1的转录本，也无COL1或COL3区域的差异。与母马不同，无论詹妮子宫内膜的分类如何，在紧凑层中总是发现不同程度的COL3，而COL1主要存在于深层基质中。随着母马中纤维化的发展，在表面上皮下臭名昭著的COL1纤维大量增加。珍妮子宫内膜中性粒细胞计数与COL1和COL3面积之间存在相关性，尽管嗜酸性粒细胞计数无相关性。在轻度病变的子宫内膜中，中性粒细胞与COL1区域呈正相关，与COL3区域呈负相关，表明珍妮子宫内膜中的中性粒细胞可能参与了纤​​维形成。另外，当嗜酸性粒细胞减少时，子宫内膜与纤维化建立反应，这可由促纤维化细胞因子IL33刺激，其释放可能归因于成纤维细胞。需要进一步的研究来分析紧致层中或子宫内膜腺周围表面上皮细胞附近的COL3对珍妮子宫内膜功能和生育能力的影响。