Locomotion speed changes appear following hippocampal injury. We used a hippocampal penetrating brain injury mouse model to analyze other kinematic changes. We found a significant decrease in locomotion speed in both open-field and tunnel walk tests. We described a new quantitative method that allows us to analyze and compare the displacement curves between mice steps. In the tunnel walk, we marked mice with indelible ink on the knee, ankle, and metatarsus of the left and right hindlimbs to evaluate both in every step. Animals with hippocampal damage exhibit slower locomotion speed in both hindlimbs. In contrast, in the cortical injured group, we observed significant speed decrease only in the right hindlimb. We found changes in the displacement patterns after hippocampal injury. Mesenchymal stem cell-derived extracellular vesicles had been used for the treatment of several diseases in animal models. Here, we evaluated the effects of intranasal administration of endometrial mesenchymal stem cell-derived extracellular vesicles on the outcome after the hippocampal injury. We report the presence of vascular endothelial growth factor, granulocyte–macrophage colony-stimulating factor, and interleukin 6 in these vesicles. We observed locomotion speed and displacement pattern preservation in mice after vesicle treatment. These mice had lower pyknotic cells percentage and a smaller damaged area in comparison with the nontreated group, probably due to angiogenesis, wound repair, and inflammation decrease. Our results build up on the evidence of the hippocampal role in walk control and suggest that the extracellular vesicles could confer neuroprotection to the damaged hippocampus.

运动速度变化在海马损伤后出现。我们使用了海马穿透性脑损伤小鼠模型来分析其他运动学变化。我们发现在野外和隧道步行测试中，运动速度都显着降低。我们描述了一种新的定量方法，使我们能够分析和比较小鼠步骤之间的位移曲线。在隧道行走中，我们在小鼠的左，右后肢的膝盖，脚踝和meta骨上用了不可磨灭的墨水标记，以便在每个步骤中进行评估。有海马损伤的动物在两个后肢中均表现出较慢的运动速度。相反，在皮层损伤组中，我们仅在右后肢观察到明显的速度下降。我们发现海马损伤后移位模式发生了变化。间充质干细胞来源的细胞外囊泡已被用于治疗动物模型中的几种疾病。在这里，我们评估了鼻内膜间充质干细胞来源的细胞外囊泡鼻内给药对海马损伤后结局的影响。我们报道了在这些囊泡中存在血管内皮生长因子，粒细胞-巨噬细胞集落刺激因子和白介素6的存在。我们观察了囊泡处理后小鼠的运动速度和位移模式的保存。与未经治疗的组相比，这些小鼠的泌尿生殖细胞百分比较低，受损区域较小，这可能是由于血管生成，伤口修复和炎症减轻所致。