The research on hatching ecology of the Chinese softshell turtle Trionyx sinensis has essential guiding roles to clarify the physiological and ecological mechanism of reptile evolution. The aim of this study is to describe the histological changes, differentiation, and maturation of some functional cells during the genesis and development of the liver and pancreas of the Chinese softshell turtle T. sinensis. Softshell turtle eggs were incubated under artificial conditions and hatched within 41-45 days. Hematoxylin and eosin-stained embryonic pancreas and liver were examined at various time points from 2 to 31 days and compared with that of other reptiles, amphibians, fishes, and birds in the literature. Immunohistochemical assay for glucagon and insulin was performed on paraformaldehyde-fixed embryos to identify functional cells in the pancreas. Pancreatic endocrine cells of T. sinensis have secretory ability at day 26 of embryonic development, and the dispersed pancreatic endocrine cells may be the result of the incomplete pancreatic development.

中文翻译：

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中华鳖胚胎肝脏和胰腺的发育

中华鳖的孵化生态研究对阐明爬行动物进化的生理生态机制具有重要的指导作用。本研究的目的是描述中华鳖肝脏和胰腺发生和发育过程中某些功能细胞的组织学变化、分化和成熟。软壳龟蛋在人工条件下孵化并在 41-45 天内孵化。在 2 至 31 天的不同时间点检查苏木精和伊红染色的胚胎胰腺和肝脏，并与文献中的其他爬行动物、两栖动物、鱼类和鸟类进行比较。对多聚甲醛固定的胚胎进行胰高血糖素和胰岛素的免疫组织化学分析，以确定胰腺中的功能细胞。