ABSTRACT

Liquidambar orientalis Mill (LOM), is an endemic species having a local distribution in the southwestern coastal district of Turkey. Styrax liquidus gum (SLG), is a gum-like resinous which exudates in response to injury of the trunk of LOM. The aim of the study was to investigate the cytotoxic effects and the molecular mechanisms of the ethanolic SLG extract in human prostate cancer cells. GC-MS analysis was performed to identify the volatile compound composition. Cytotoxicity was determined by XTT analysis. Apoptosis and necrosis were evaluated via ELISA assay. Autophagic cell death was detected via monodansylcadaverine (MDC) staining and by measuring the levels of LC3I and LC3II. The protein levels of p-PI3K, p-Akt and p-mTOR were evaluated by western blot analysis. In the present study, it is shown that the SLG extract containing a considerable amount of ravidomycin derivate induced autophagic cell death in prostate cancer cells via inhibiting the PI3K/Akt/mTOR pathway.

摘要

东方枫香磨坊（LOM）是一种地方性物种，分布于土耳其西南沿海地区。Styrax liquidus gum (SLG) 是一种胶状树脂，在 LOM 的躯干损伤时会渗出。该研究的目的是研究乙醇 SLG 提取物在人前列腺癌细胞中的细胞毒性作用和分子机制。进行 GC-MS 分析以确定挥发性化合物的组成。通过 XTT 分析确定细胞毒性。通过ELISA测定评估细胞凋亡和坏死。通过单丹磺酰尸胺 (MDC) 染色和测量 LC3I 和 LC3II 的水平来检测自噬性细胞死亡。通过蛋白质印迹分析评估 p-PI3K、p-Akt 和 p-mTOR 的蛋白质水平。在目前的研究中，