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Genome‐wide analysis of mitogen‐activated protein ( MAP ) kinase gene family expression in response to biotic and abiotic stresses in sugarcane
Physiologia Plantarum ( IF 6.4 ) Pub Date : 2020-09-28 , DOI: 10.1111/ppl.13208 Ahmad Ali 1, 2 , Na Chu 1, 2 , Panpan Ma 2, 3 , Talha Javed 1, 2 , Uroosa Zaheer 1, 2 , Mei-Ting Huang 1 , Hua-Ying Fu 1 , San-Ji Gao 1
Physiologia Plantarum ( IF 6.4 ) Pub Date : 2020-09-28 , DOI: 10.1111/ppl.13208 Ahmad Ali 1, 2 , Na Chu 1, 2 , Panpan Ma 2, 3 , Talha Javed 1, 2 , Uroosa Zaheer 1, 2 , Mei-Ting Huang 1 , Hua-Ying Fu 1 , San-Ji Gao 1
Affiliation
To systematically analyze mitogen-activated protein (MAP) kinase gene families and their expression profiles in sugarcane (Saccharum spp. hybrids; Sh) under diverse biotic and abiotic stresses, we identified 15 ShMAPKs, 6 ShMAPKKs, and 16 ShMAPKKKs genes in the sugarcane cultivar R570 genome. These were also confirmed in one S. spontaneum genome and two transcriptome datasets of sugarcane trigged by Acidovorax avenae subsp. avenae (Aaa) and Xanthomonas albilineans (Xa) infections. Phylogenetic analysis revealed that four subgroups were present in each ShMAPK and ShMAPKK family and three sub-families (RAF, MEKK, and ZIK) presented in the ShMAPKKK family. Conserved protein motif and gene structure analyses supported the evolutionary relationships of the three families inferred from the phylogenetic analysis. All of the ShMAPK, ShMAPKK, and ShMAPKKK genes identified in Saccharum spp. R570 were distributed on chromosomes 1-7 and 9-10. RNA-seq and qRT-PCR analyses indicated that ShMAPK07 and ShMAPKKK02 were defense-responsive genes in sugarcane challenged by both Aaa and Xa stimuli, while some genes were up-regulated specifically by Aaa and Xa infection. Additionally, ShMAPK05 acted as a negative regulator under drought and salinity stress, but served as a positive regulator under salicylic acid (SA) treatment. ShMAPK07 plays a positive role under drought stress, but a negative role under SA treatment. ShMAPKKK01 was negatively modulated by both salinity stress and SA treatment, whereas ShMAPKKK06 was positively regulated by both of the two stress stimuli. Our results suggest that members of MAPK cascade gene families regulate adverse stress responses through multiple signal transduction pathways in sugarcane. This article is protected by copyright. All rights reserved.
中文翻译:
响应甘蔗生物和非生物胁迫的丝裂原活化蛋白(MAP)激酶基因家族表达的全基因组分析
为了系统地分析在不同生物和非生物胁迫下甘蔗(Saccharum spp. hybrids; Sh)中丝裂原活化蛋白 (MAP) 激酶基因家族及其表达谱,我们在甘蔗品种中鉴定了 15 个 ShMAPKs、6 个 ShMAPKKs 和 16 个 ShMAPKKKs 基因R570 基因组。这些也在一个 S. spontaneum 基因组和两个由 Acidovorax avenae subsp 触发的甘蔗转录组数据集中得到证实。avenae (Aaa) 和 Xanthomonas albilineans (Xa) 感染。系统发育分析显示,每个 ShMAPK 和 ShMAPKK 家族中存在四个亚群,ShMAPKKK 家族中存在三个亚家族(RAF、MEKK 和 ZIK)。保守的蛋白质基序和基因结构分析支持从系统发育分析中推断出的三个家族的进化关系。所有的 ShMAPK、ShMAPKK、和 ShMAPKKK 基因在 Saccharum spp 中鉴定。R570 分布在 1-7 和 9-10 号染色体上。RNA-seq 和 qRT-PCR 分析表明 ShMAPK07 和 ShMAPKKK02 是甘蔗中受到 Aaa 和 Xa 刺激物攻击的防御反应基因,而一些基因被 Aaa 和 Xa 感染特异性上调。此外,ShMAPK05 在干旱和盐度胁迫下充当负调节剂,但在水杨酸 (SA) 处理下充当正调节剂。ShMAPK07在干旱胁迫下起积极作用,但在SA处理下起消极作用。ShMAPKKK01 受到盐度胁迫和 SA 处理的负调节,而 ShMAPKKK06 受到两种胁迫刺激的正调节。我们的研究结果表明,MAPK 级联基因家族的成员通过甘蔗中的多种信号转导途径调节不利的应激反应。本文受版权保护。版权所有。
更新日期:2020-09-28
中文翻译:
响应甘蔗生物和非生物胁迫的丝裂原活化蛋白(MAP)激酶基因家族表达的全基因组分析
为了系统地分析在不同生物和非生物胁迫下甘蔗(Saccharum spp. hybrids; Sh)中丝裂原活化蛋白 (MAP) 激酶基因家族及其表达谱,我们在甘蔗品种中鉴定了 15 个 ShMAPKs、6 个 ShMAPKKs 和 16 个 ShMAPKKKs 基因R570 基因组。这些也在一个 S. spontaneum 基因组和两个由 Acidovorax avenae subsp 触发的甘蔗转录组数据集中得到证实。avenae (Aaa) 和 Xanthomonas albilineans (Xa) 感染。系统发育分析显示,每个 ShMAPK 和 ShMAPKK 家族中存在四个亚群,ShMAPKKK 家族中存在三个亚家族(RAF、MEKK 和 ZIK)。保守的蛋白质基序和基因结构分析支持从系统发育分析中推断出的三个家族的进化关系。所有的 ShMAPK、ShMAPKK、和 ShMAPKKK 基因在 Saccharum spp 中鉴定。R570 分布在 1-7 和 9-10 号染色体上。RNA-seq 和 qRT-PCR 分析表明 ShMAPK07 和 ShMAPKKK02 是甘蔗中受到 Aaa 和 Xa 刺激物攻击的防御反应基因,而一些基因被 Aaa 和 Xa 感染特异性上调。此外,ShMAPK05 在干旱和盐度胁迫下充当负调节剂,但在水杨酸 (SA) 处理下充当正调节剂。ShMAPK07在干旱胁迫下起积极作用,但在SA处理下起消极作用。ShMAPKKK01 受到盐度胁迫和 SA 处理的负调节,而 ShMAPKKK06 受到两种胁迫刺激的正调节。我们的研究结果表明,MAPK 级联基因家族的成员通过甘蔗中的多种信号转导途径调节不利的应激反应。本文受版权保护。版权所有。