Salmonella enterica serovar Typhimurium (S. Typhimurium) is exposed to biotic and abiotic stresses. The survival of Salmonella in nature depends on the global regulators like cAMP receptor protein (CRP). The role of CRP in the phenotypic characteristics and stress tolerance was elucidated in S. Typhimurium using a crp gene null mutant (Δcrp). A 1.6‐fold decrease in the cell size, a two‐fold reduction in the colony size, and a 3.5‐fold decrease in motility were observed in the Δcrp compared with the S. Typhimurium wild‐type (WT). H₂S production on selective media was affected in the Δcrp. The utilisation of d‐mannose, d‐glycerol and d‐mannitol was completely affected, whereas that of d‐galactose and d‐fructose was partially affected. The utilisation of d‐arabinose was induced in the Δcrp. The growth rate of the Δcrp in Luria Bertani medium was unaffected. However, in the glucose‐containing minimal medium, the growth rate of the Δcrp was reduced by 1.5‐fold compared with the WT. The Δcrp was able to utilise ethanolamine as the sole carbon source similar to the WT. The Δcrp was more tolerant to heat and oxidative stress. Overexpression of heat and oxidative stress‐related genes was observed in the Δcrp in the stationary phase. The Δcrp was less tolerant to radiation stress compared with the WT. The current findings decisively establish the CRP protein as a global regulator. The CRP affects multiple phenotypes, carbon metabolism and stress physiology of S. Typhimurium.

中文翻译：

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cAMP受体蛋白在肠炎沙门氏菌鼠伤寒沙门氏菌表型和应激耐受性中的作用。

沙门氏菌血清型鼠伤寒沙门氏菌（S. Typhimurium）暴露于生物和非生物胁迫。沙门氏菌在自然界中的生存取决于诸如cAMP受体蛋白（CRP）等全局调节因子。CRP在表型特征和抗逆性的作用被阐明小号。鼠伤寒使用crp基因无效突变体（Δcrp）。与S相比，Δcrp的细胞大小减少了1.6倍，菌落大小减少了2倍，运动能力减少了3.5倍。鼠伤寒野生型（WT）。选择性介质上的H ₂ S产生受到Δcrp的影响。的利用率d甘露糖，d -甘油和d -mannitol完全的影响，而其的d半乳糖和d -fructose部分地受到影响。的利用率d -arabinose在Δ诱导CRP。在Δ的增长率CRP在Luria Bertani培养基未受影响。但是，在含葡萄糖的基本培养基中，与野生型相比，Δcrp的生长速率降低了1.5倍。该Δ CRP是能够利用乙醇胺作为类似于WT唯一碳源。该Δ CRP更耐高温和氧化应激。在固定相的Δcrp中观察到热和氧化应激相关基因的过表达。该Δ CRP是宽容少到辐射应力与WT相比较。目前的发现决定性地将CRP蛋白确立为全球监管者。CRP影响S的多种表型，碳代谢和应激生理。鼠伤寒