The enzymatic protein hydrolysates of Mytilus coruscus (MCPH) were prepared with 5 proteases. The low molecular weight (MW) peptide fractions (<1 kDa, MCPH-T1) of trypsin hydrolysates were obtained using ultrafiltration. MCPH-T1 showed the most competitive DPPH•, ABTS⁺•, OH•, and O₂⁻• scavenging activities compared to other enzymatic hydrolysates or high MW fractions. Compared to glutathione, MCPH-T1 showed better ferric ion-reducing power and inhibited lipid peroxidation of linoleic acid. In H₂O₂-induced oxidative injured of human umbilical vein endothelial cells (HUVEC), MCPH-T1 pre-treatment improved cell viability and ameliorated the morphological damage through attenuating the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) production and restored the cellular antioxidant capacities. In addition, MCPH-T1 showed a cytoprotective effect on dysfunctional cell by regulating the expressions of apoptotic and NF-κB proteins and restored the Nrf2-driven antioxidant defense mechanism. These results indicated that MCPH-T1 may be potential food ingredients for managing oxidative stress-related endothelial dysfunction or related cardiovascular diseases.

用5种蛋白酶制备了Mytilus coruscus（MCPH）的酶蛋白水解物。胰蛋白酶水解物的低分子量（MW）肽级分（<1 kDa，MCPH-T1）使用超滤技术获得。MCPH-T1显示出最有竞争力的DPPH•，ABTS ⁺ •，OH•和O ₂ ^-相对于其它酶水解产物或高MW馏分•清除活性。与谷胱甘肽相比，MCPH-T1表现出更好的铁离子还原能力，并抑制了亚油酸的脂质过氧化。在H ₂ O _2中诱导的人脐静脉内皮细胞（HUVEC）氧化损伤，MCPH-T1预处理通过减弱活性氧（ROS）和丙二醛（MDA）的积累来改善细胞活力并减轻形态损伤，并恢复细胞抗氧化剂能力。此外，MCPH-T1通过调节凋亡和NF-κB蛋白的表达对功能障碍的细胞显示出细胞保护作用，并恢复了Nrf2驱动的抗氧化防御机制。这些结果表明，MCPH-T1可能是治疗氧化应激相关的内皮功能障碍或相关心血管疾病的潜在食品成分。