Constructed wetlands form a unique ecosystem having plants, soil, microbes in which microorganisms play a vital role in the transformation and degradation of pollutants from wastewater. In the present study, French type two-stage vertical flow constructed wetland (VFCW) was used for the treatment of single household greywater (GW). Pilot-scale VFCW having sand and gravel as the filter substrate was constructed with Canna indica plantation for treating GW. To understand the pollutant removal mechanism in VFCW, microbial diversity and functional annotation was carried out by metagenomics analysis of sequences obtained from illumina platform. Efficiency of VFCW was measured with respect to water quality parameters like COD, BOD5, Total Nitrogen, Nitrate, Nitrite, Ammoniacal-N, ortho-phosphate and TOC from inlet and outlet of system. The removal efficiency was 90%, 93%, 34%, 26%, 89%, 68%, 80%, and 80% for COD, BOD5, Total Nitrogen, Nitrate, Nitrite, Ammoniacal-N, ortho-phosphate and TOC respectively. Microbial diversity was much more diversified and unique in VFCW compared to GW. Metagenomes exhibited Proteobacteria and Bacteroidetes as major phyla in GW whereas Actinobacteria, Proteobacteria, Nitrospirae abundance in VFCW layers. Total of 809 and 695 genus were found in VFCW and GW respectively with minimum abundance of 10 hits. From functional annotation of sequences, VFCW microbes have the potential to transform various aromatic and xenobiotic compounds along with the removal of pollutants present in the form of Carbon, Nitrogen, and Phosphorus. These data reveal French type VFCW can efficiently treat GW and with its own unique, variable habitat VFCW harbours diverse community of microorganisms that transform and degrade the pollutants in GW.

中文翻译：

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法国垂直流人工湿地处理灰水微生物多样性及功能注释

人工湿地形成了一个独特的生态系统，其中包含植物、土壤和微生物，其中微生物在废水中污染物的转化和降解中起着至关重要的作用。在本研究中，法式两级垂直流人工湿地（VFCW）用于处理单户灰水（GW）。以砂石为过滤基质的中试规模VFCW 是用美人蕉种植园建造的，用于处理GW。为了了解 VFCW 中的污染物去除机制，通过对从 illumina 平台获得的序列进行宏基因组学分析，进行微生物多样性和功能注释。VFCW 的效率是根据水质参数如 COD、BOD5、总氮、硝酸盐、亚硝酸盐、氨氮、正磷酸盐和系统入口和出口的 TOC 来测量的。对COD、BOD5、总氮、硝酸盐、亚硝酸盐、氨氮、正磷酸盐和TOC的去除效率分别为90%、93%、34%、26%、89%、68%、80%和80% . 与 GW 相比，VFCW 中的微生物多样性更加多样化和独特。宏基因组显示变形菌门和拟杆菌门是 GW 中的主要门，而放线菌、变形菌门和 VFCW 层中的硝化螺菌属丰度。在 VFCW 和 GW 中分别发现了 809 和 695 个属，最小丰度为 10 个命中。从序列的功能注释来看，VFCW 微生物有可能转化各种芳香族和异生物质，同时去除以碳、氮和磷形式存在的污染物。这些数据揭示了法式 VFCW 可以有效治疗 GW 并以其独特的、