Cultured peritoneal macrophages from intact (control) and BCG-infected (experiment) male BALB/c mice were studied 90 days after infection. Polarization of macrophages by M1 (expression of GM-CSF, IFNγ, and CD16/32) and M2 (expression of bFGF and CD36) differentiation pathways was studied with consideration for their the nuclearity class. Mononuclear cells predominated (90% and higher) in macrophage cultures of both groups and presumably, were presented by mainly epithelioid cells. The results indicated polarization of mononuclear and multinuclear macrophages in the M2 direction under conditions of BCG granulomatosis and a higher initial M2 polarization of binuclear macrophages. In control cultures, the ratio of M2 to M1 macrophages was 0.57, in experimental cultures this ratio was 1.6. It seems that long persistence of Mycobacterium tuberculosis in macrophages served as a factor stimulating the plastic processes and transformation of macrophages into epithelioid cells that form the "core" of granulomas and their enlargement upon incorporation of macrophages.

中文翻译：

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BCG 感染小鼠巨噬细胞的体外 M1/M2 极化

感染后 90 天研究了来自完整（对照）和 BCG 感染（实验）雄性 BALB/c 小鼠的培养的腹膜巨噬细胞。研究了 M1（表达 GM-CSF、IFNγ 和 CD16/32）和 M2（表达 bFGF 和 CD36）分化途径对巨噬细胞的极化，同时考虑了它们的核类型。单核细胞在两组的巨噬细胞培养物中占主导地位（90% 或更高），并且可能主要由上皮样细胞呈递。结果表明，在 BCG 肉芽肿条件下，单核和多核巨噬细胞在 M2 方向极化，双核巨噬细胞初始 M2 极化较高。在对照培养物中，M2 与 M1 巨噬细胞的比率为 0.57，在实验培养物中该比率为 1.6。