We studied the effect of echinochrome A on the primary culture of pulmonary fibroblasts under normal conditions and under oxidative stress. Exposure to echinochrome A (100 μM, 6 h) did not affect the production of superoxide radical by fibroblasts, area of their nuclei, and number of nucleoli, but reduced the total area of nucleolar organizer regions against the background of stable proliferative activity of the culture. Two-hour oxidative stress induced by hydrogen peroxide solution (60 μM) increased the generation of superoxide radical, decreased DNA-synthetic activity of fibroblasts, area of fibroblast nuclei, and total area of nucleolar organizer regions. Preliminary administration of echinochrome A significantly attenuated the damaging effect of oxidative stress: the intensity of production of superoxide radicals decreased, DNA-synthetic activity and nucleus area of fibroblasts partially recovered; normalization of the total area of nucleoli was accompanied by an increase in their number.

中文翻译：

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Echinochrome A在氧化应激条件下白化大鼠肺成纤维细胞原代培养中的细胞保护作用

我们研究了棘突色素 A 在正常条件下和氧化应激下对肺成纤维细胞原代培养的影响。暴露于棘突色素 A（100 μM，6 小时）不会影响成纤维细胞产生超氧自由基、其核面积和核仁数量，但在稳定增殖活性的背景下减少了核仁组织者区域的总面积文化。由过氧化氢溶液 (60 μM) 诱导的 2 小时氧化应激增加了超氧自由基的产生，降低了成纤维细胞的 DNA 合成活性、成纤维细胞核面积和核仁组织区域的总面积。棘突色素 A 的初步给药显着减弱了氧化应激的破坏作用：超氧自由基的产生强度降低，成纤维细胞的DNA合成活性和细胞核面积部分恢复；核仁总面积的正常化伴随着它们数量的增加。