A novel chemiluminescent method was developed to evaluate ROS generation by platelets. This method allows measuring activities of NADPH oxidase (NOX2) and enzymes synthesizing secondary ROS (superoxide dismutase, catalase, etc.) in resting and ADP-activated platelets (inductor of platelet aggregation and ROS generation) using a small number of cells. The method was tested in the examination of patients with coronary heart disease. It was found that platelets from patients with coronary heart disease were characterized by NOX2 activation, while cell metabolism is tuned for a long-term intensive production of superoxide anion radical. The enzymes synthesizing secondary ROS were also activated, but cell metabolism could not maintain their enhanced activity for a long time.

中文翻译：

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冠心病患者血小板合成活性氧的化学发光分析

开发了一种新的化学发光方法来评估血小板产生的 ROS。该方法允许使用少量细胞测量静息和 ADP 激活的血小板（血小板聚集和 ROS 生成的诱导剂）中 NADPH 氧化酶 (NOX2) 和合成二次 ROS（超氧化物歧化酶、过氧化氢酶等）的酶的活性。该方法在冠心病患者的检查中进行了测试。研究发现，冠心病患者的血小板以 NOX2 激活为特征，而细胞代谢则被调整为长期大量产生超氧阴离子自由基。合成次级ROS的酶也被激活，但细胞代谢不能长时间保持其增强的活性。