Antigen delivery systems play critical roles in determining the quality and quantity of Ab responses in vivo. Induction of protective antibodies by B cells is essential in the development of vaccines against infectious pathogens, whereas production of IgE antibodies is prerequisite for investigation of allergic responses, or type 1 hypersensitivity reactions. Virus-like particles (VLPs) are efficient platforms for expression of proteins of interest in highly repetitive manners, which grants strong Ab responses to target antigens. Here, we report that delivery of hen egg lysozyme (HEL), a model allergen, through VLP could provoke strong HEL specific IgE Ab responses in mice. Moreover, acute allergic responses were robustly induced in the mice sensitized with VLPs that express HEL, when challenged with recombinant HEL protein. Our data show that antigen delivery in the context of VLPs could function as a platform for sensitization of mice and for subsequent examination of allergic reactions to molecules of interest.

中文翻译：

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病毒样颗粒 (VLP) 介导的抗原递送作为实验过敏小鼠模型的致敏工具

抗原递送系统在确定体内抗体反应的质量和数量方面发挥着关键作用。B 细胞诱导保护性抗体对于开发针对传染性病原体的疫苗至关重要，而 IgE 抗体的产生是研究过敏反应或 1 型超敏反应的先决条件。病毒样颗粒 (VLP) 是以高度重复的方式表达感兴趣的蛋白质的有效平台，可对目标抗原产生强烈的抗体反应。在这里，我们报告说，通过 VLP 传递模型过敏原鸡蛋溶菌酶 (HEL) 可以在小鼠中引起强烈的 HEL 特异性 IgE Ab 反应。此外，当用重组 HEL 蛋白进行攻击时，在用表达 HEL 的 VLP 致敏的小鼠中强烈诱导了急性过敏反应。