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Cytogenomic characterization of three murine malignant mesothelioma tumor cell lines.
Molecular Cytogenetics ( IF 1.3 ) Pub Date : 2020-09-09 , DOI: 10.1186/s13039-020-00511-4
Eva Wahlbuhl 1 , Thomas Liehr 1 , Martina Rincic 2 , Shaymaa Azawi 1
Affiliation  

Malignant mesothelioma (MM) is a rare aggressive cancer primary located in pleura and lung. MMs can be divided into biphasic, epithelioid and sarcomatoid subtypes. In majority of cases MMs are induced by asbestos fiber exposure. As latency period after asbestos exposure ranges between ~ 10 and 60 years MMs are mainly observed in elder people. Human MM, being a rare tumor type, lacks detailed cytogenetic data, while molecular genetic studies have been undertaken more frequently. However, murine MM cell lines are also regularly applied to get more insight into MM biology and to test new therapy strategies. Here the murine MM cell lines AB1, AB22 and AC29 were studied by molecular cytogenetics and molecular karyotyping. Interestingly, yet there were no genetic or genomic studies undertaken for these already in 1992 established cell lines. The obtained data on genomic imbalances in these murine cell lines was translated into the human genome as previously reported based on human and murine genomic browsers. It turned out that all three cell lines showed high similarities in copy number variants as observed typically in human MM. Also, all three cell lines were most similar to human epithelioid MMs, and should be used as models therefore.

中文翻译:

三种鼠恶性间皮瘤肿瘤细胞系的细胞基因组学特征。

恶性间皮瘤 (MM) 是一种罕见的侵袭性癌症,原发于胸膜和肺。MM可分为双相、上皮样和肉瘤样亚型。在大多数情况下,MMs 是由石棉纤维暴露引起的。由于石棉暴露后的潜伏期介于约 10 至 60 年之间,MMs 主要在老年人中观察到。人类 MM 是一种罕见的肿瘤类型,缺乏详细的细胞遗传学数据,而分子遗传学研究的开展更为频繁。然而,鼠 MM 细胞系也经常用于更深入地了解 MM 生物学并测试新的治疗策略。在这里,通过分子细胞遗传学和分子核型分析研究了鼠 MM 细胞系 AB1、AB22 和 AC29。有趣的是,1992 年已经建立的细胞系尚未对这些细胞系进行遗传或基因组研究。如先前基于人类和鼠基因组浏览器所报道的,所获得的关于这些鼠细胞系中基因组失衡的数据被转化为人类基因组。事实证明,所有三种细胞系在拷贝数变异方面都表现出高度相似性,这通常在人类 MM 中观察到。此外,所有三种细胞系都与人类上皮样 MM 最相似,因此应用作模型。
更新日期:2020-09-10
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