Polysaccharides, with an extensive distribution in natural products, represent a group of natural bioactive substances having widespread applications in health-care food products and as biomaterials. Devising an efficient system for the separation and purification of polysaccharides from natural sources, hence, is of utmost importance in the widespread applicability and feasibility of research for the development of polysaccharide-based products. High-speed counter-current chromatography (HSCCC) is a continuous liquid–liquid partitioning chromatography with the ability to support a high loading amount and crude material treatment. Due to its flexible two-phase solvent system, HSCCC has been successfully used in the separation of many natural products. Based on HSCCC unique advantages over general column chromatography and its enhanced superiority in this regard when coupled to aqueous two-phase system (ATPS), this review summarizes the separation and purification of various bioactive polysaccharides through HSCCC and its coupling to ATPS as an aid in future research in this direction.

中文翻译：

---

高速逆流色谱法分离纯化生物活性多糖的研究进展。

在天然产物中广泛分布的多糖代表了一组天然生物活性物质，在保健食品和生物材料中得到广泛应用。因此，设计用于从天然来源分离和纯化多糖的有效系统对于开发基于多糖的产品的广泛应用和可行性研究至关重要。高速逆流色谱法（HSCCC）是一种连续的液-液分配色谱法，能够支持高载量和粗物质处理。由于其灵活的两相溶剂系统，HSCCC已成功用于许多天然产物的分离。