Abstract

Background

Grain mills, feed mills, ethanol plants, flour producers, but also mobile laboratory services need simple and reliable possibilities to analyze grain for mycotoxins during harvest in the field.

Objective

The RIDA^®SMART APP is a smartphone application, which was developed together with a Lateral Flow Device as a quantitative and fast on-site/on-field tool for mycotoxin analyses.

Method

It is a small and light device with its own power supply, very versatile, flexible and easy to use. For quantification, a lot specific calibation curve is uploaded to the RIDA^®SMART APP via a QR code from the certificate of analysis. The software application in combination with a compatible smartphone, analyzes the LFD strip via a picture taken with a smartphone and quantifies the mycotoxin contamination within 4 second. Due to the possibility to use mobile networks or WIFI, there are no difficulties in the organization of the collected results. Currently, nine different android-based smartphones from various manufacturers are compatible with the RIDA^®SMART APP.

Results

In combination with one of the compatible smartphones, the RIDA^®SMART APP is able to generate results with a coefficient of variation CV ≤ 13%, even at highly varying lighting conditions. The recovery of certified reference corn samples, previously analyzed with HPLC or LC-MS/MS, shows a mean value between 91% - 119%.

Conclusion

The RIDA^®SMART APP is highly suitable for an inexpensive, quick and accurate quantitative measurement of mycotoxins in agricultural products (e.g. aflatoxins in corn).

Highlights

To our best knowledge, the RIDA^®SMART APP is the only smartphone-based method for quantitative mycotoxin analysis worldwide combined with a broader measuring range as most usual lateral flow analyzers.

中文翻译：

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下一代 LFD 测试 – 使用 RIDA®SMART APP 进行基于智能手机的霉菌毒素分析

摘要

背景

谷物加工厂、饲料加工厂、乙醇厂、面粉生产商以及移动实验室服务都需要简单可靠的方法来分析谷物在田间收获期间的霉菌毒素。

客观的

RIDA ^® SMART APP 是一款智能手机应用程序，它与侧流设备一起开发，作为用于真菌毒素分析的定量和快速现场/现场工具。

方法

它是一种小巧轻便的设备，自带电源，用途广泛，灵活且易于使用。为了量化，很多特定的校准曲线通过分析证书中的二维码上传到 RIDA ^® SMART APP。该软件应用程序与兼容的智能手机相结合，通过智能手机拍摄的照片分析 LFD 条，并在 4 秒内量化真菌毒素污染。由于可以使用移动网络或WIFI，因此收集结果的组织不存在困难。目前，来自不同制造商的九款不同的基于安卓的智能手机与 RIDA ^® SMART APP兼容。

结果

结合其中一款兼容的智能手机，RIDA ^® SMART APP 能够生成变异系数 CV ≤ 13% 的结果，即使在高度变化的光照条件下也是如此。先前使用 HPLC 或 LC-MS/MS 分析的认证参考玉米样品的回收率显示平均值在 91% - 119% 之间。

结论

RIDA ^® SMART APP 非常适合对农产品中的霉菌毒素（例如玉米中的黄曲霉毒素）进行廉价、快速和准确的定量测量。

强调

据我们所知，RIDA ^® SMART APP 是全球唯一一种基于智能手机的真菌毒素定量分析方法，与最常用的侧流分析仪一样具有更广泛的测量范围。