SummaryTo investigate the roles of lncRNA deleted in lymphocytic leukaemia 1 (DLEU1) on migration and invasion of human trophoblast cells. Human chorionic trophoblast cell line HTR8/SVneo was cultured and transfected using lncRNA DLEU1 small interfering RNA. Real-time quantitative polymerase chain reaction was used to detect lncRNA DLEU1 expression. The activity of migration regulatory protein CDC42 was detected by western blot. The downstream miRNA targets of lncRNA and mRNAs targeted by corresponding miRNAs were respectively predicted using bioinformatics analyses. Compared with the control group, the expression of lncRNA DLEU1 in the small interfering RNA group was significantly decreased (P < 0.05). There was no significant change in cell proliferation capacity for transfected cells (lncRNA DLEU1 siRNA-1, P = 0.537; lncRNA DLEU1 siRNA-2, P = 0.384), but cell migration (lncRNA DLEU1 siRNA-1, P = 0.025; lncRNA DLEU1 siRNA-2, P = 0.019) and invasion (lncRNA DLEU1 siRNA-1, P = 0.0327; lncRNA DLEU1 siRNA-2, P = 0.021) was significantly reduced. CDC42 activity in the lncRNA DLEU1 knockdown group decreased and the phosphorylation of cofilin increased. Therefore, downregulation of lncRNA DLEU1 suppressed the migration and invasion of human trophoblast cells.

中文翻译：

---

淋巴细胞白血病1（DLEU1）中缺失的lncRNA促进人胚胎滋养层细胞的迁移和侵袭

摘要研究淋巴细胞白血病1（DLEU1）中缺失的lncRNA对人滋养层细胞迁移和侵袭的作用。使用 lncRNA DLEU1 小干扰 RNA 培养和转染人绒毛膜滋养层细胞系 HTR8/SVneo。实时定量聚合酶链反应用于检测lncRNA DLEU1的表达。Western blot检测迁移调节蛋白CDC42的活性。使用生物信息学分析分别预测lncRNA的下游miRNA靶标和相应miRNA靶向的mRNA。与对照组相比，小干扰RNA组lncRNA DLEU1的表达明显降低（磷 <0.05)。转染细胞的细胞增殖能力没有显着变化（lncRNA DLEU1 siRNA-1，磷= 0.537; lncRNA DLEU1 siRNA-2，磷= 0.384），但细胞迁移（lncRNA DLEU1 siRNA-1，磷= 0.025; lncRNA DLEU1 siRNA-2，磷= 0.019) 和入侵 (lncRNA DLEU1 siRNA-1,磷= 0.0327; lncRNA DLEU1 siRNA-2，磷= 0.021) 显着降低。lncRNA DLEU1 敲低组中 CDC42 活性降低，cofilin 磷酸化增加。因此，lncRNA DLEU1的下调抑制了人滋养层细胞的迁移和侵袭。