Introduction: Ischemic stroke is the third leading cause of death. There is no known treatment or cure for the disease. Moreover, the pathological mechanism of ischemic stroke remains unclear. Objective: We aimed to identify potential microRNAs (miRNAs) and mRNAs, contributing to understanding the pathology of ischemic stroke. Methods: First, the data of miRNA and mRNA were downloaded for differential expression analysis. Then, the regulatory network between miRNA and mRNAs was constructed. Third, top 100 differentially expressed mRNAs were used to construct a protein-protein interaction network followed by the function annotation of mRNAs. In addition, in vitro experiment was used to validate the expression of mRNAs. Last, receiver operating characteristic diagnostic analysis of differentially methylated genes was performed. Results: Totally, up to 26 differentially expressed miRNAs and 1,345 differentially expressed mRNAs were identified. Several regulatory interaction pairs between miRNA and mRNAs were identified, such as hsa-miR-206-HMGCR/PICALM, hsa-miR-4491-TMEM97, hsa-miR-3622b-5p/hsa-miR-548k-KLF12, and hsa-miR-302a-3p/hsa-miR-3145-3p-CTSS. MAPK signaling pathway (involved DUSP1) and the Notch signaling pathway (involved NUMB and CREBBP) were identified. The expression validation of KLF12, ARG1, ITGAM, SIRT4, SERPINH1, and DUSP1 was consistent with the bioinformatics analysis. Interestingly, hsa-miR-206, hsa-miR-4491, hsa-miR-3622b-5p, hsa-miR-548k, hsa-miR-302a-3p, hsa-miR-3145-3p, KLF12, and ID3 had the potential diagnostic value of ischemic stroke. Conclusions: The identified differentially expressed miRNAs and mRNAs may be associated with the development of ischemic stroke.

中文翻译：

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基于基因表达综合数据库中的综合分析，揭示缺血性中风中潜在的差异表达 miRNA 和 mRNA

简介：缺血性中风是第三大死亡原因。这种疾病没有已知的治疗方法或治愈方法。此外，缺血性脑卒中的病理机制尚不清楚。目的：我们旨在识别潜在的 microRNAs (miRNAs) 和 mRNAs，有助于了解缺血性中风的病理。方法：首先下载miRNA和mRNA的数据进行差异表达分析。然后，构建了miRNA和mRNA之间的调控网络。第三，利用前100个差异表达的mRNA构建蛋白质-蛋白质相互作用网络，然后对mRNA进行功能注释。此外，体外实验用于验证mRNA的表达。最后，对差异甲基化基因进行受试者操作特征诊断分析。结果：总共，鉴定了多达 26 个差异表达的 miRNA 和 1,345 个差异表达的 mRNA。确定了 miRNA 和 mRNA 之间的几个调控相互作用对，例如 hsa-miR-206-HMGCR/PICALM、hsa-miR-4491-TMEM97、hsa-miR-3622b-5p/hsa-miR-548k-KLF12 和 hsa- miR-302a-3p/hsa-miR-3145-3p-CTSS。确定了 MAPK 信号通路（涉及 DUSP1）和 Notch 信号通路（涉及 NUMB 和 CREBBP）。KLF12、ARG1、ITGAM、SIRT4、SERPINH1和DUSP1的表达验证与生物信息学分析一致。有趣的是，hsa-miR-206、hsa-miR-4491、hsa-miR-3622b-5p、hsa-miR-548k、hsa-miR-302a-3p、hsa-miR-3145-3p、KLF12和ID3具有缺血性卒中的潜在诊断价值。结论：鉴定出的差异表达miRNAs和mRNAs可能与缺血性卒中的发生发展有关。