Recent reports have pointed to the link between persistent inflammation, oxidative stress, and carcinogenesis; however most of the studies concerning the role of viruses in head and neck cancer (HNC) are focused mainly on one type of virus. Our present study aimed to study the relationship between Epstein–Barr virus/human papilloma virus (EBV/HPV) coinfection and glutathione peroxidase (GPx) and superoxide dismutase (SOD) level in oropharyngeal cancer. Fresh-frozen tumor tissue samples were collected from 128 patients with oropharyngeal cancer infected with EBV or HPV or with EBV/HPV coinfection. After DNA extraction, EBV and HPV DNA was detected using a polymerase chain reaction (PCR) assay. GPx and SOD activity was determined in homogenates of cancer tissue using diagnostic kits produced by Randox Laboratories. Both GPx and SOD activity was statistically lower in patients with EBV/HPV coinfection than in a single EBV or HPV infection. Analysis of GPx and SOD activity in relation to histological grading and tumor, node (TN) classification revealed that in poorly-differentiated tumors, the level of antioxidant enzymes was lower compared with well-differentiated lesions and in cases with greater tumor dimensions and lymph-node involvement, both GPx and SOD activity was decreased. Further studies are necessary to clarify the influence of interplay between EBV, HPV, and oxidative stress on malignant transformation of upper aerodigestive tract epithelial cells.

中文翻译：

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EBV和HPV合并感染的口咽癌中的谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）。

最近的报道指出了持续性炎症，氧化应激和致癌之间的联系。然而，大多数有关病毒在头颈癌（HNC）中作用的研究主要集中在一种病毒上。我们目前的研究旨在研究口咽癌中爱泼斯坦-巴尔病毒/人乳头瘤病毒（EBV / HPV）共感染与谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）水平之间的关系。从128例被EBV或HPV或EBV / HPV合并感染的口咽癌患者中收集新鲜冷冻的肿瘤组织样本。DNA提取后，使用聚合酶链反应（PCR）分析检测EBV和HPV DNA。使用Randox Laboratories生产的诊断试剂盒测定癌组织匀浆中的GPx和SOD活性。EBV / HPV合并感染患者的GPx和SOD活性均低于单一EBV或HPV感染患者。对GPx和SOD活性与组织学分级和肿瘤，淋巴结（TN）分类相关的分析表明，在分化较差的肿瘤中，抗氧化酶的水平低于分化良好的病灶，并且在具有较大肿瘤尺寸和淋巴结转移的情况下节点参与，GPx和SOD活性均降低。有必要进行进一步的研究来阐明EBV，HPV和氧化应激之间的相互作用对上消化道上皮细胞恶性转化的影响。淋巴结（TN）分类显示，在分化较差的肿瘤中，抗氧化酶的水平低于分化良好的病灶；在肿瘤尺寸较大且淋巴结受累的情况下，GPx和SOD活性均降低。有必要进行进一步的研究来阐明EBV，HPV和氧化应激之间的相互作用对上消化道上皮细胞恶性转化的影响。淋巴结（TN）分类显示，在分化较差的肿瘤中，抗氧化酶的水平低于分化良好的病灶；在肿瘤尺寸较大且淋巴结受累的情况下，GPx和SOD活性均降低。有必要进行进一步的研究来阐明EBV，HPV和氧化应激之间的相互作用对上消化道上皮细胞恶性转化的影响。