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Nocardamine-dependent iron uptake in Pseudomonas aeruginosa: exclusive involvement of the FoxA outer membrane transporter.
ACS Chemical Biology ( IF 4 ) Pub Date : 2020-09-09 , DOI: 10.1021/acschembio.0c00535
Vincent Normant 1 , Inokentijs Josts 2, 3 , Lauriane Kuhn 4 , Quentin Perraud 1 , Sarah Fritsch 1 , Philippe Hammann 4 , Gaëtan L A Mislin 1 , Henning Tidow 2, 3 , Isabelle J Schalk 1
Affiliation  

Iron is a key nutrient for almost all living organisms. Paradoxically, it is poorly soluble and consequently poorly bioavailable. Bacteria have thus developed multiple strategies to access this metal. One of the most common consists of the use of siderophores, small compounds that chelate ferric iron with very high affinity. Many bacteria are able to produce their own siderophores or use those produced by other microorganisms (exosiderophores) in a piracy strategy. Pseudomonas aeruginosa produces two siderophores, pyoverdine and pyochelin, and is also able to use a large panel of exosiderophores. We investigated the ability of P. aeruginosa to use nocardamine (NOCA) and ferrioxamine B (DFOB) as exosiderophores under iron-limited planktonic growth conditions. Proteomic and RT-qPCR approaches showed induction of the transcription and expression of the outer membrane transporter FoxA in the presence of NOCA or DFOB in the bacterial environment. Expression of the proteins of the heme- or pyoverdine- and pyochelin-dependent iron uptake pathways was not affected by the presence of these two tris-hydroxamate siderophores. 55Fe uptake assays using foxA mutants showed ferri-NOCA to be exclusively transported by FoxA, whereas ferri-DFOB was transported by FoxA and at least one other unidentified transporter. The crystal structure of FoxA complexed with NOCA-Fe revealed very similar siderophore binding sites between NOCA-Fe and DFOB-Fe. We discuss iron uptake by hydroxamate exosiderophores in P. aeruginosa cells in light of these results.

中文翻译:

铜绿假单胞菌中依赖诺卡胺的铁摄取:FoxA外膜转运蛋白的独家参与。

铁是几乎所有活生物体的关键营养素。矛盾的是,它溶解性差,因此生物利用度差。细菌因此开发出多种策略来获取这种金属。最常见的方法之一是使用铁载体,即以非常高的亲和力螯合三价铁的小化合物。许多细菌能够产生自己的铁载体或在盗版策略中使用其他微生物产生的铁载体。铜绿假单胞菌产生两种铁载体,丙酮丁酮和pyochelin,并且还能够使用一大批外铁载体。我们调查了铜绿假单胞菌的能力在铁限制的浮游生物生长条件下,使用去甲酚胺(NOCA)和亚铁胺B(DFOB)作为外铁载体。蛋白质组学和RT-qPCR方法显示在细菌环境中存在NOCA或DFOB的情况下诱导外膜转运蛋白FoxA的转录和表达。这两个tris-异羟肟酸酯铁载体的存在不影响血红素或pyoverdine和pyyochelin依赖性铁摄取途径的蛋白质的表达。使用foxA进行55种铁吸收测定突变体显示Ferri-NOCA仅由FoxA转运,而ferri-DFOB由FoxA和至少一个其他身份不明的转运蛋白转运。与NOCA-Fe络合的FoxA的晶体结构揭示了NOCA-Fe和DFOB-Fe之间非常相似的铁载体结合位点。根据这些结果,我们讨论了异羟肟酸酯外铁载体在铜绿假单胞菌细胞中的铁吸收。
更新日期:2020-10-17
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