Abstract

Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying. Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm. The composite films have a porosity of 95.3%, and showed good hydrophilicity with swelling ratio of 19 ± 1.8 and in vitro degradability. X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film. The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay. Thus, the nano-biocomposite films of BC-C-G could be of paramount importance as tissue engineering scaffold. The “all-natural” ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.

摘要

细菌纤维素-壳聚糖-明胶 (BC-CG) 三元纳米生物复合膜是通过将 BC 薄膜浸入壳聚糖和明胶混合物中并随后冷冻干燥来制备的。纳米生物复合膜的扫描电子显微镜 (SEM) 图像显示存在相互连接的孔，纤维直径为 20-150 nm。复合膜的孔隙率为95.3%，并表现出良好的亲水性，溶胀比为19±1.8 ，体外降解性。X 射线衍射、衰减全反射傅里叶变换红外光谱和热重 (TG) 分析结果显示薄膜内的 BC、明胶和壳聚糖分子之间存在一些相互作用。复合膜为 L929 成纤维细胞的粘附和增殖提供了良好的基质，如细胞附着研究、细胞膜构建体的 FE-SEM 和细胞相容性测定所示。因此，BC-CG 的纳米生物复合膜作为组织工程支架可能是最重要的。BC-CG 的“全天然”三元聚合物复合膜之前尚未针对生物医学应用进行评估。