Abstract

Due to their health-promoting effects green tea catechins have gained a keen interest in recent years in the context of bodyweight reduction treatments and alleviation of inflammatory diseases. This study was designed to evaluate the impact of native and thermally modified catechins (TMC) on the body weight gain, fatty acid profile in subcutaneous adipose tissue and the activity of the enzymes involved in lipid metabolism regulation: AMP-dependent protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in apoE-deficient mice maintained on a high-fat diet. We observed that TMC decreased bodyweight gain as compared to the control group. Furthermore, TMC increased AMPK activity and reduced ACC activity in the metabolically important tissues: intestine, liver and subcutaneous adipose tissue and affected adipose tissue fatty acid composition. Native catechins produced less pronounced effects. These results suggest that TMC down-regulate endogenous fatty acid synthesis, which should be taken into account in dietary applications of catechins.

摘要

由于其促进健康的作用，绿茶儿茶素近年来在减轻体重治疗和缓解炎症疾病的背景下引起了浓厚的兴趣。本研究旨在评估天然和热改性儿茶素 (TMC) 对体重增加、皮下脂肪组织中的脂肪酸谱以及参与脂质代谢调节的酶活性的影响：AMP 依赖性蛋白激酶 (AMPK)和乙酰辅酶A羧化酶（ACC）在维持高脂肪饮食的apoE缺陷小鼠中。我们观察到与对照组相比，TMC 降低了体重增加。此外，TMC 增加了代谢重要组织中的 AMPK 活性并降低了 ACC 活性：肠道、肝脏和皮下脂肪组织，并影响了脂肪组织的脂肪酸组成。天然儿茶素产生的效果不太明显。这些结果表明 TMC 下调内源性脂肪酸合成，这应该在儿茶素的膳食应用中加以考虑。