ABSTRACT

1. In this study, the proteomics method was applied to genetically evaluate the performance and carcase characteristics of Japanese quail and their molecular mechanisms, and to further determine and analyse the meat quality and muscle proteins.

2. Ten Japanese quail were selected from among 400 quail from the seventh generation of a population. Various traits were recorded and multivariate analysis was applied using Wombat software.

3. Differential expression of pectoral muscle proteins was performed, whereby nine spots were selected (P < 0.05) for determination. All proteins from the quail group with the highest breeding value showed significantly greater relative intensity, except for serum albumin.

4. The results showed that an increase in growth rate can cause disturbances in most organs and their metabolism, although the increase in the expression of some proteins indicated that the bird’s body tends to adapt to special conditions.

5. The allocation of genetic resources is likely to maintain the balance between most organs, which does not overlap. However, most evidence shows that meat quality has been reduced, but to a limited extent, by selection for growth.

摘要

1.本研究采用蛋白质组学方法对日本鹌鹑的性能和case体特征及其分子机制进行遗传评估，并进一步确定和分析肉的品质和肌肉蛋白质。

2.从第七代人口的400只鹌鹑中选出10只日本鹌鹑。记录各种性状，并使用Wombat软件进行多元分析。

3.进行胸肌蛋白的差异表达，从而选择九个斑点（P ＜0.05）进行测定。鹌鹑组中所有具有最高繁殖价值的蛋白质，除血清白蛋白外，均显示出相对更高的相对强度。

4.结果表明，虽然某些蛋白质的表达增加表明鸟类的身体倾向于适应特殊条件，但生长速率的增加可能会导致大多数器官及其代谢紊乱。

5.遗传资源的分配很可能维持大多数器官之间的平衡，这种平衡并不重叠。但是，大多数证据表明，通过选择生长，肉质已经降低，但是在一定程度上有所降低。