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The risk of unintentional propagation of poliovirus can be minimized by using human cell lines lacking the functional CD155 gene.
Microbiology and Immunology ( IF 2.6 ) Pub Date : 2020-09-09 , DOI: 10.1111/1348-0421.12843
Satoshi Koike 1
Affiliation  

After eradication and containment of wild poliovirus (PV) and cessation of oral polio vaccinations, it is critical to minimize the risk of reintroducing PV into PV‐free communities via facilities that handle the virus. The potential risk of unintentional PV propagation through unidentified contaminated materials is a serious issue. This study reports the generation of HeLa and RD‐A cells deficient in functional CD155 gene (∆PVR cells); these cells are not susceptible to PV but remain susceptible to other picornaviruses. These ∆PVR cells will minimize the risk of unintentional transmission of PV and support performing the experiments more safely.

中文翻译:

通过使用缺少功能性CD155基因的人类细胞系,可以将脊髓灰质炎病毒意外传播的风险降到最低。

在消灭和遏制野生脊髓灰质炎病毒(PV)并停止口服脊髓灰质炎疫苗后,至关重要的是,必须通过处理病毒的设施将PV重新引入无PV社区的风险降至最低。光伏通过不明身份的污染材料意外传播的潜在风险是一个严重的问题。这项研究报告了功能性CD155基因缺陷的HeLa和RD-A细胞(∆PVR细胞)的产生;这些细胞对PV不敏感,但对其他小核糖核酸病毒仍然敏感。这些∆PVR电池可最大程度减少意外传播PV的风险,并支持更安全地进行实验。
更新日期:2020-09-09
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