Nest building is an instinctive behavior toward protection from predators, body temperature regulation, and courtship. Previously, we discovered that acute and chronic social defeat stress suppresses the onset of nest‐building behavior in male mice (C57BL/6J). Here, we analyzed nest building and other behavioral deficits induced by acute social defeat stress (ASDS). We utilized a customized cage and specifically developed observational programs for nest building, social avoidance, and other behaviors using an infrared depth camera to acquire three‐dimensional (3D) data of animal behavior (Negura system). We determined the volume of nesting materials from these 3D depth images. Mice exposed to ASDS showed increased spontaneous activities, decreased rearing, and delayed nest building; however, nest‐building activity was gradually recovered during the dark period of the 24 hr observation interval. At the endpoint following 24 hr, the ASDS and control groups showed no differences in nest volumes. Furthermore, we observed the time courses of both nest building and social avoidance behaviors and their relationship using the Negura system. Our data demonstrated a weak positive correlation between nest‐building delay and social avoidance in ASDS mice. The Negura system can observe various behaviors that reflect the effects of social defeat stress.

中文翻译：

---

使用三维深度相机评估暴露于急性社交失败压力的小鼠的筑巢和社交互动行为。

筑巢是一种保护免受捕食者、调节体温和求爱的本能行为。此前，我们发现急性和慢性社交失败压力会抑制雄性小鼠 (C57BL/6J) 筑巢行为的发生。在这里，我们分析了由急性社交失败压力 (ASDS) 引起的筑巢和其他行为缺陷。我们使用定制的笼子，并专门开发了用于筑巢、社交回避和其他行为的观察程序，使用红外深度相机获取动物行为的三维 (3D) 数据（Negura 系统）。我们根据这些 3D 深度图像确定了嵌套材料的体积。暴露于 ASDS 的小鼠表现出自发活动增加、饲养减少和筑巢延迟；然而，在 24 小时观察间隔的黑暗时期，筑巢活动逐渐恢复。在 24 小时后的终点，ASDS 和对照组的巢体积没有差异。此外，我们使用 Negura 系统观察了筑巢和社交回避行为的时间进程及其关系。我们的数据表明 ASDS 小鼠的筑巢延迟和社交回避之间呈弱正相关。Negura 系统可以观察反映社会失败压力影响的各种行为。我们的数据表明 ASDS 小鼠的筑巢延迟和社交回避之间呈弱正相关。Negura 系统可以观察反映社会失败压力影响的各种行为。我们的数据表明 ASDS 小鼠的筑巢延迟和社交回避之间呈弱正相关。Negura 系统可以观察反映社会失败压力影响的各种行为。