Anti-müllerian hormone (AMH) is an established marker of ovarian reserve that decreases with age. Though the pool of ovarian follicles is established during fetal development, impacts of in utero exposures on AMH are uncertain. Thus, we sought to evaluate associations of in utero exposures with AMH of adult daughters with a prospective cohort study of adult daughters at university medical centers. Women noted their mother’s reported use of diethylstilbestrol (DES), vitamins, tobacco, alcohol, and caffeine during pregnancy, and their mother’s occupation during pregnancy. All participants were reproductive age women (18-40 years) enrolled in the Effects of Aspirin in Gestation and Reproduction (EAGeR) trial. Serum AMH concentrations were measured at baseline prior to conception and categorized using clinical guidelines. Multinomial regression models estimated associations between each exposure and high (>3.5 ng/mL) and low (<1.0 ng/mL) versus normal AMH (1.0-3.5 ng/mL), adjusting for participant’s age, mother’s age, mother’s history of fertility treatment, and mother’s use of vitamins. In 1202 women with available data, maternal caffeine use was associated with an increased risk of low AMH, compared to normal (relative risk [RR] 1.90, 95% confidence interval [CI] 1.09, 3.30). Vitamins were associated with an increased risk of high AMH compared to normal (RR 1.93, 95% CI 1.24, 3.00). Other exposures were not associated with AMH concentrations in offspring. Maternal caffeine and vitamin use during pregnancy may be associated with ovarian reserve in adult offspring, highlighting the potential importance of pregnancy lifestyle on the reproductive health of daughters.

抗苗勒管激素 (AMH) 是一种成熟的卵巢储备标志物，随着年龄的增长而下降。虽然卵泡池是在胎儿发育期间建立的，但子宫内暴露对 AMH 的影响尚不确定。因此，我们试图评估子宫内在大学医疗中心对成年女儿进行前瞻性队列研究，以了解成年女儿的 AMH 暴露情况。妇女注意到她们的母亲报告在怀孕期间使用己烯雌酚 (DES)、维生素、烟草、酒精和咖啡因，以及她们母亲在怀孕期间的职业。所有参与者都是育龄女性（18-40 岁），参加了阿司匹林对妊娠和生殖的影响 (EAGeR) 试验。在受孕前在基线测量血清 AMH 浓度，并使用临床指南进行分类。多项回归模型估计了每次暴露与高 (>3.5 ng/mL) 和低 (<1.0 ng/mL) 与正常 AMH (1.0-3.5 ng/mL) 之间的关联，并根据参与者的年龄、母亲的年龄、母亲的生育史进行调整治疗，以及母亲对维生素的使用。在有可用数据的 1202 名妇女中，与正常人相比，母亲使用咖啡因与低 AMH 的风险增加有关（相对风险 [RR] 1.90，95% 置信区间 [CI] 1.09, 3.30）。与正常相比，维生素与高 AMH 风险增加相关（RR 1.93，95% CI 1.24，3.00）。其他暴露与后代的 AMH 浓度无关。母亲在怀孕期间使用咖啡因和维生素可能与成年后代的卵巢储备有关，突出了怀孕生活方式对女儿生殖健康的潜在重要性。其他暴露与后代的 AMH 浓度无关。母亲在怀孕期间使用咖啡因和维生素可能与成年后代的卵巢储备有关，突出了怀孕生活方式对女儿生殖健康的潜在重要性。其他暴露与后代的 AMH 浓度无关。母亲在怀孕期间使用咖啡因和维生素可能与成年后代的卵巢储备有关，突出了怀孕生活方式对女儿生殖健康的潜在重要性。