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Detecting cadmium during ultrastructural characterization of hepatotoxicity.
Journal of Trace Elements in Medicine and Biology ( IF 3.5 ) Pub Date : 2020-09-09 , DOI: 10.1016/j.jtemb.2020.126644
Kuo Qi 1 , Longfei Ren 2 , Zhongtian Bai 3 , Jun Yan 4 , Xia Deng 5 , Jianjun Zhang 6 , Yong Peng 7 , Xun Li 8
Affiliation  

Background

The threat of cadmium (Cd), which is the cause of itai-itai disease in Japan, is still complicated and confusing, especially for digestive system, such as liver disease. One of the most keys of this problem is demonstrating that the hepatotoxicity is indeed induced by Cd. Therefore, we attempt detecting Cd at microscale during ultrastructural imaging of liver tissue.

Methods

12 rats were divided randomly into two experimental groups: control and Cd-treated. Treated rats were intraperitoneal injected with 1 mg/kg body weight cadmium chloride (CdCl2) for 4 weeks (5 P.M each day for 6 days/week). At the end of the exposure period, liver tissue samples were processed into ultrathin sections for analysis of advanced analytical transmission electron microscopy and X-ray energy dispersive spectroscopy (TEM/X-EDS) investigations. Ultrastructural images and X-ray energy dispersive spectrum were acquired at microscale.

Results

Cd can cause changes in the structure of the organelle, including the collapse of the membrane structure in the cell, the destruction of the internal structure of the organelle, the mitochondrial swelling, the expansion of the endoplasmic reticulum, and the appearance of inclusions. Cadmium bioaccumulation is detected in the mitochondria at microscale by TEM/X-EDS, which is the visual evidence of morphological changes of mitochondria related to Cd.

Conclusion

The combination of detailed ultrastructure and microscale X-ray energy dispersive spectroscopy (X-EDS) characterization of cadmium hepatotoxicity demonstrate that cadmium indeed leads to mitochondrial damage, which is helpful for further investigation of the pathological mechanism of cadmium hepatotoxicity.



中文翻译:

在肝毒性超微结构表征过程中检测镉。

背景

镉(Cd)的威胁在日本是引起痛痛病的原因,但仍然是复杂和令人困惑的,尤其是对消化系统,如肝脏疾病。这个问题的最关键之一是证明肝毒性确实是由 Cd 引起的。因此,我们尝试在肝组织的超微结构成像过程中以微尺度检测 Cd。

方法

12 只大鼠随机分为两个实验组:对照组和 Cd 治疗组。用1mg/kg体重的氯化镉(CdCl 2 )腹膜内注射经处理的大鼠4周(每天下午5点,持续6天/周)。在暴露期结束时,肝组织样本被加工成超薄切片,用于高级分析透射电子显微镜和 X 射线能量色散光谱 (TEM/X-EDS) 研究。在微尺度上获得超微结构图像和 X 射线能量色散谱。

结果

Cd可引起细胞器结构的改变,包括细胞内膜结构的塌陷、细胞器内部结构的破坏、线粒体肿胀、内质网扩张、包涵体的出现等。通过TEM/X-EDS在微米尺度上检测到线粒体中镉的生物蓄积,这是与Cd相关的线粒体形态变化的视觉证据。

结论

镉肝毒性的详细超微结构和微尺度 X 射线能量色散谱 (X-EDS) 表征的结合表明,镉确实会导致线粒体损伤,这有助于进一步研究镉肝毒性的病理机制。

更新日期:2020-09-18
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