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The Mitochondria/Caspase-Dependent Apoptotic Pathway Plays a Role in the Positive Effects of a Power frequency electromagnetic field on Alzheimer’s Disease Neuronal Model
Journal of Chemical Neuroanatomy ( IF 2.8 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.jchemneu.2020.101857 Hongyan Zuo 1 , Xiao Liu 2 , Yang Li 3 , Dewen Wang 1 , Yanhui Hao 1 , Chao Yu 1 , Xinping Xu 1 , Ruiyun Peng 1 , Tao Song 4
Journal of Chemical Neuroanatomy ( IF 2.8 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.jchemneu.2020.101857 Hongyan Zuo 1 , Xiao Liu 2 , Yang Li 3 , Dewen Wang 1 , Yanhui Hao 1 , Chao Yu 1 , Xinping Xu 1 , Ruiyun Peng 1 , Tao Song 4
Affiliation
In this study, rat pheochromocytoma (PC12) cells were induced into an Alzheimer's Disease (AD) neuronal model using nerve growth factor (NGF; 50 ng/mL) and Amyloid β25-35 (20 μmol/L). Changes in the morphological structure, cell viability, apoptosis rate, and expression of apoptosis-related protein induced by exposure to a power frequency electromagnetic field (PF-MF; 50 Hz, 100 µT, 24 h) were detected respectively by light and electron microscopy, the MTT assay, immunohistochemistry, flow cytometry and enzyme-linked immunosorbent assays. The results showed that 3-12 h after PF-MF exposure, the pathological injury was improved partly; metabolic activity was promoted and cell apoptosis was inhibited in the AD neuronal model. In addition, PF-MF exposure significantly inhibited the expression of Caspase8, Caspase3, and CytC, but increased the Bcl-2/Bax ratio of the AD neuronal model. Meanwhile, PF-MF seemed to have no effect on the expression of Fas and TNFR1. This study indicated that the mitochondria/caspase-dependent apoptotic pathway plays an important role in the positive effects of PF-MF on an AD neuronal model. The results suggested that PF-MF exposure might have potential therapeutic value for AD, and the underling molecular mechanisms still need further studies.
中文翻译:
线粒体/半胱天冬酶依赖性凋亡通路在工频电磁场对阿尔茨海默病神经元模型的积极影响中发挥作用
在本研究中,使用神经生长因子 (NGF;50 ng/mL) 和淀粉样蛋白 25-35 (20 μmol/L) 将大鼠嗜铬细胞瘤 (PC12) 细胞诱导为阿尔茨海默病 (AD) 神经元模型。通过光镜和电镜分别检测工频电磁场(PF-MF;50 Hz,100 µT,24 h)诱导的形态结构、细胞活力、凋亡率和凋亡相关蛋白表达的变化、MTT 测定、免疫组织化学、流式细胞术和酶联免疫吸附测定。结果表明PF-MF暴露3-12h后,病理损伤得到部分改善;AD神经元模型中代谢活性得到促进,细胞凋亡受到抑制。此外,PF-MF 暴露显着抑制 Caspase8、Caspase3 和 CytC 的表达,但增加了 AD 神经元模型的 Bcl-2/Bax 比率。同时,PF-MF 似乎对 Fas 和 TNFR1 的表达没有影响。该研究表明,线粒体/半胱天冬酶依赖性细胞凋亡途径在 PF-MF 对 AD 神经元模型的积极影响中起着重要作用。结果表明,PF-MF 暴露可能对 AD 具有潜在的治疗价值,潜在的分子机制仍需进一步研究。
更新日期:2020-11-01
中文翻译:
线粒体/半胱天冬酶依赖性凋亡通路在工频电磁场对阿尔茨海默病神经元模型的积极影响中发挥作用
在本研究中,使用神经生长因子 (NGF;50 ng/mL) 和淀粉样蛋白 25-35 (20 μmol/L) 将大鼠嗜铬细胞瘤 (PC12) 细胞诱导为阿尔茨海默病 (AD) 神经元模型。通过光镜和电镜分别检测工频电磁场(PF-MF;50 Hz,100 µT,24 h)诱导的形态结构、细胞活力、凋亡率和凋亡相关蛋白表达的变化、MTT 测定、免疫组织化学、流式细胞术和酶联免疫吸附测定。结果表明PF-MF暴露3-12h后,病理损伤得到部分改善;AD神经元模型中代谢活性得到促进,细胞凋亡受到抑制。此外,PF-MF 暴露显着抑制 Caspase8、Caspase3 和 CytC 的表达,但增加了 AD 神经元模型的 Bcl-2/Bax 比率。同时,PF-MF 似乎对 Fas 和 TNFR1 的表达没有影响。该研究表明,线粒体/半胱天冬酶依赖性细胞凋亡途径在 PF-MF 对 AD 神经元模型的积极影响中起着重要作用。结果表明,PF-MF 暴露可能对 AD 具有潜在的治疗价值,潜在的分子机制仍需进一步研究。