Myst family genes encode lysine acetyltransferases that mainly mediate histone acetylation to control transcription, DNA replication and DNA damage response. They form tetrameric complexes with PHD-finger proteins (Brpfs or Jades) and small non-catalytic subunits Ing4/5 and Meaf6. Although all the components of the complex are well-conserved from yeast to mammals, the function of Meaf6 and its homologs has not been elucidated in any species. Here we revealed the role of Meaf6 utilizing inducible Meaf6 KO ES cells. By elimination of Meaf6, proliferation ceased although histone acetylations were largely unaffected. In the absence of Meaf6, one of the Myst family members Myst2/Kat7 increased the ability to interact with PHD-finger proteins. This study is the first indication of the function of Meaf6, which shows it is not essential for HAT activity but modulates the assembly of the Kat7 complex.

Myst 家族基因编码主要介导组蛋白乙酰化以控制转录、DNA 复制和 DNA 损伤反应的赖氨酸乙酰转移酶。它们与 PHD 指蛋白（Brpfs 或 Jades）和小的非催化亚基 Ing4/5 和 Meaf6 形成四聚体复合物。尽管该复合物的所有成分从酵母到哺乳动物都保存完好，但尚未在任何物种中阐明 Meaf6 及其同源物的功能。在这里，我们揭示了 Meaf6 利用诱导型Meaf6 KO ES 细胞的作用。通过消除Meaf6，尽管组蛋白乙酰化在很大程度上不受影响，但增殖停止了。在没有 Meaf6 的情况下，Myst 家族成员之一 Myst2/Kat7 增加了与 PHD 指蛋白相互作用的能力。这项研究首次表明了 Meaf6 的功能，这表明它对 HAT 活动不是必需的，但可以调节 Kat7 复合物的组装。