Purpose

This study aimed to elucidate the effect of preheat-treatments and bleaching agents on molecular characteristics and bioavailability of obtained bio-calcium.

Methods

Bio-calcium from Asian sea bass backbone was prepared using different preheat-treatment methods (boiling at 100 °C for 30 min or autoclaving at 121 °C for 90 min). Subsequent bleaching was carried out using 2.5% sodium hypochlorite, followed by 2.5% hydrogen peroxide or using 2.5% hydrogen peroxide alone.

Results

Bio-calcium powder from autoclaved bone had higher calcium content (35.9 ± 0.2%) than that produced from boiled bone (25.7 ± 1.2%). Particle size of bio-calcium from autoclaved bone (16.71 ± 16.71 µm) was lower than that from boiled bone (52.85 ± 43.16 µm). Highest whiteness index (92.26 ± 0.21) was observed for autoclaved bone bio-calcium when hydrogen peroxide alone was used as bleaching agent. FTIR study confirmed that Ca-hydroxyapatite was present in both bio-calcium from boiled and autoclaved bones but the amount was higher in the latter.

Conclusion

Boiling or boiling/autoclaving could be alternative pre-treatment for bio-calcium production to reduce chemical or alkali used and to soften the bone. However, solubility of autoclaved bone bio-calcium was lower than boiled bone counterpart. Enhancement of solubility is still needed to improve bio-availability of bio-calcium from Asian sea bass backbone.

Graphic Abstract

中文翻译：

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预热处理和漂白剂对亚洲鲈鱼骨钙生物钙的影响

目的

这项研究旨在阐明预热处理和漂白剂对获得的生物钙的分子特性和生物利用度的影响。

方法

使用不同的预热处理方法（在100°C下沸腾30分钟或在121°C下高压灭菌90分钟）制备来自亚洲鲈鱼骨干的生物钙。随后的漂白使用2.5％的次氯酸钠，然后2.5％的过氧化氢或单独使用2.5％的过氧化氢进行。

结果

高压灭菌骨中的生物钙粉的钙含量（35.9±0.2％）比沸腾骨中的钙含量（25.7±1.2％）高。高压灭菌骨中生物钙的粒径（16.71±16.71 µm）比沸腾骨中生物钙的粒径（52.85±43.16 µm）低。当单独使用过氧化氢作为漂白剂时，高压灭菌的骨生物钙的白度指数最高（92.26±0.21）。FTIR研究证实，煮沸和高压灭菌的骨头中的生物钙中都存在钙-羟基磷灰石，但后者中的含量更高。

结论

煮沸或煮沸/高压灭菌可以作为生物钙生产的替代预处理，以减少化学物质或碱的使用并软化骨骼。但是，高压灭菌的骨生物钙的溶解度低于煮沸的骨生物钙。仍需要增强溶解度以改善亚洲鲈鱼骨干中生物钙的生物利用度。

图形摘要