Molecular markers are considered powerful tools to identify the potential genetic variation. Forty-two RAPD, ISSR and SSR markers were employed to characterize 21 mungbean genotypes. RAPD primers produced 79 polymorphic bands while ISSR and SSR markers amplified 21 and 6 polymorphic bands, respectively. The range for minimum and maximum number of bands was 3–13, 3–9 and 1–2 and average alleles per loci were 8.17, 4.3 and 1 for RAPD, ISSR and SSR, respectively. Highest polymorphism percentage was 100% for both ISSR and SSR while 80% for RAPD markers. The SSR-VR-303 and ISSR-UBC-810 had highest PIC values (0.44, 0.72) indicating the more discriminating power of these primers for diversity analysis. RAPD primer OPA-7 with maximum PIC value (0.26) resulted in good amplification. Jaccard’s similarity coefficient ranged between 0.50 to 1, 0.64 to 1 and 0.75 to 1 for SSR, ISSR and RAPD primers, respectively, indicating less genetic divergence among studied material. Dendrogram based on Unweighted Pair Group Method of Arithmetic Means (UPGMA) grouped mungbean genotypes into two to three major clusters for different marker system with up to 100% genetic relatedness among different cultivars. Molecular genetic divergence identified can be utilized to widen the genetic base in mungbean breeding programs.

分子标记被认为是识别潜在遗传变异的有力工具。42个RAPD，ISSR和SSR标记用于表征21个绿豆基因型。RAPD引物产生79条多态性条带，而ISSR和SSR标记分别扩增21条和6条多态性条带。RAPD，ISSR和SSR的最小和最大条带范围分别为3-13、3-9和1-2，每个基因座的平均等位基因分别为8.17、4.3和1。ISSR和SSR的最高多态性百分比均为100％，而RAPD标记为80％。SSR-VR-303和ISSR-UBC-810的PIC值最高（0.44、0.72），表明这些引物对多样性的分析能力更高。具有最大PIC值（0.26）的RAPD引物OPA-7具有良好的扩增效果。Jaccard的相似系数介于0.50到1,0之间。SSR，ISSR和RAPD引物分别为64：1和0.75：1，表明研究材料之间的遗传差异较小。基于算术平均非加权对（UPGMA）的树状图将绿豆基因型分为两到三个主要簇，用于不同的标记系统，不同品种间的遗传相关性高达100％。鉴定出的分子遗传差异可用于扩大绿豆育种计划的遗传基础。