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SARS-CoV-2 envelope protein topology in eukaryotic membranes.
Open Biology ( IF 5.8 ) Pub Date : 2020-09-09 , DOI: 10.1098/rsob.200209
Gerard Duart 1 , Mª Jesús García-Murria 1 , Brayan Grau 1 , José M Acosta-Cáceres 1 , Luis Martínez-Gil 1 , Ismael Mingarro 1
Affiliation  

Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Ntlum/Ctcyt). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and host components and contribute to establish the basis to tackle the pathogenesis of SARS-CoV-2.



中文翻译:

真核细胞膜中的SARS-CoV-2包膜蛋白拓扑。

冠状病毒E蛋白是一种在病毒包膜中发现的小膜蛋白。不同的冠状病毒E蛋白具有惊人的生化和功能相似性,但序列保守性受到限制。在本报告中,我们研究了新的SARS-CoV-2病毒在微粒体膜和哺乳动物细胞中的E蛋白拓扑。实验数据表明,E蛋白是单跨膜蛋白,N端跨膜易位,而C端暴露于细胞质侧(Nt lum / Ct cyt)。定义的SARS-CoV-2 E蛋白膜蛋白拓扑结构可提供一个有用的框架,以了解其与其他病毒和宿主成分的相互作用,并有助于建立解决SARS-CoV-2发病机理的基础。

更新日期:2020-09-09
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