The current research was undertaken to determine the potency of two distinct probiotics Biogut® Aqua (feed probiotic) and Bioclean® Aqua (water and soil probiotic) to induce immunity and protection faced due to Vibrio pathogen during cultivation of Mozambique tilapia under controlled laboratory trials. Assessment was carried out using immune gene expression and immunological assays. During the initial phase of treatment, nitro blue tetrazolium (NBT) assay, antiprotease assay and peroxidase assay displayed a convincing raise in serum activity of the probiotic managed feed when compared to the control groups. In addition, qPCR real time analysis displayed in which nutritional implementation with feed probiotic vitally increased the interpretation of immune related genes, particularly TNF and MCP gene expression which are involved in immune response. The results revealed that the probiotics Bioclean® Aqua and Biogut® Aqua have beneficial effects through efficiently enhancing immune system and protection in tilapia model facing Vibriosis. Hence it can be stated that the application of probiotics like Biogut® Aqua and Bioclean® Aqua in aquaculture environment may significantly reduce the antibiotic usage, thereby preventing the entry of these chemicals into the food chain. Additionally it was demonstrated that the ammonia level in water can be reduced significantly with the application of Bioclean® Aqua (water and soil probiotic). The toxicity studies proved that both the probiotic are non-toxic and safe for the use in aquaculture environment.

中文翻译：

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商业用益生菌在罗非鱼中诱导免疫应答和抵抗弧菌感染的功效研究

当前的研究是为了确定两种截然不同的益生菌Biogut®Aqua（饲料益生菌）和Bioclean®Aqua（水和土壤益生菌）诱导由弧菌引起的免疫力和保护的效力。莫桑比克罗非鱼在受控实验室试验过程中的致病菌。使用免疫基因表达和免疫测定法进行评估。在治疗的初始阶段，与对照组相比，硝基蓝四唑（NBT）测定，抗蛋白酶测定和过氧化物酶测定显示了益生菌管理饲料的血清活性令人信服地提高。此外，qPCR实时分析显示，饲料中益生菌的营养实施极大地提高了免疫相关基因的解释，特别是涉及免疫应答的TNF和MCP基因表达。结果表明，益生菌Bioclean®Aqua和Biogut®Aqua通过有效增强免疫系统和保护面对弧菌病的罗非鱼模型具有有益的作用。因此可以说，在水产养殖环境中使用益生菌（如Biogut®Aqua和Bioclean®Aqua）可以显着减少抗生素的使用，从而防止这些化学物质进入食物链。此外，通过使用Bioclean®Aqua（水和土壤益生菌）可以显着降低水中的氨含量。毒性研究证明，这两种益生菌在水产养殖环境中均无毒且安全。此外，通过使用Bioclean®Aqua（水和土壤益生菌）可以显着降低水中的氨含量。毒性研究证明，这两种益生菌在水产养殖环境中均无毒且安全。此外，通过使用Bioclean®Aqua（水和土壤益生菌）可以显着降低水中的氨含量。毒性研究证明，这两种益生菌在水产养殖环境中均无毒且安全。