Plant cells contain groups of biomolecules that participate together in a particular biological process. Exogenous codelivery of multiple biomolecules is an essential step for elucidation of the biological significance of these molecules and enables various biotechnological applications in plants. However, the currently existing biomolecule delivery methods face difficulties in delivering multiple components into plant cells, mediating transgene expression, and maintaining the stability of the numerous components and lead to delays in biomolecular function. Cell-penetrating peptides (CPPs) have demonstrated remarkable abilities to introduce diverse biomolecules into various plant species. Here, we employed the engineered CPP KH₉-BP100 as a carrier to deliver multiple biomolecules into plant cells and performed a bimolecular fluorescence complementation assay to assess the simultaneous introduction of multiple biomolecules. We demonstrate that multiple biomolecule/CPP cargos can be simultaneously internalized by a particular plant cell, albeit with different efficiencies. We present a cutting-edge technique for codelivery of multiple biomolecules into plant cells that can be used for elucidation of functional correlations and for metabolic engineering.

中文翻译：

---

使用细胞穿透肽纳米载体将多种生物大分子同时引入植物细胞。

植物细胞包含一起参与特定生物过程的生物分子组。多种生物分子的外源代码传递是阐明这些分子生物学意义的必不可少的步骤，并使植物中的各种生物技术应用成为可能。然而，当前存在的生物分子递送方法在将多种组分递送到植物细胞中，介导转基因表达以及维持众多组分的稳定性以及导致生物分子功能的延迟方面面临困难。细胞穿透肽（CPP）具有将多种生物分子引入各种植物物种的出色能力。在这里，我们采用了工程CPP KH ₉-BP100作为载体将多种生物分子传递到植物细胞中，并进行了双分子荧光互补分析以评估多种生物分子的同时引入。我们证明，尽管具有不同的效率，但特定的植物细胞可以同时内化多种生物分子/ CPP货物。我们提出了一种先进的技术，可将多种生物分子代码传递到植物细胞中，可用于阐明功能相关性和进行代谢工程。