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The DNA controllable peroxidase mimetic activity of MoS2 nanosheets for constructing a robust colorimetric biosensor.
Nanoscale ( IF 6.7 ) Pub Date : 2020-09-08 , DOI: 10.1039/d0nr05649a
Lianjing Zhao 1 , Jing Wang 1 , Dandan Su 1 , Yueying Zhang 1 , Huiying Lu 1 , Xu Yan 1 , Jihao Bai 1 , Yuan Gao 1 , Geyu Lu 1
Affiliation  

The low activity of nanozymes, which work as an alternative to natural enzymes, limits their applications in the fabrication of biosensors, drawing increasing attention aimed at improving their catalytic capacity. In this work, the peroxidase-like activity of MoS2 nanosheets (NSs) was dramatically enhanced through DNA modification, and was 4.3-times higher than that of bare MoS2 NSs. Such an enhancement of catalytic activity was mainly ascribed to the increased affinity of the DNA/MoS2 NSs toward the substrate, TMB, further accelerating electron transfer from TMB to H2O2. On the basis of DNA-tuned MoS2 NS nanozyme activity, a colorimetric sensing platform was developed for the facile detection of carcinoembryonic antigen (CEA) in a sensitive manner. Interestingly, a convenient, affordable, and instrument-free portable test kit was fabricated to visually monitor CEA via rooting the aptamer/MoS2 NS system into an agarose hydrogel. Importantly, our work illuminates the feasibility of using DNA to enhance the catalysis of nanozymes and their application potential in the label-free, portable, and visual detection of aptamer-targeted biomolecules.

中文翻译:

MoS2纳米片的DNA可控过氧化物酶模拟活性,用于构建鲁棒的比色生物传感器。

纳米酶的低活性可替代天然酶,限制了它们在生物传感器制造中的应用,从而引起人们越来越多的关注,以提高其催化能力。在这项工作中,MoS 2纳米片(NSs)的过氧化物酶样活性通过DNA修饰得到了显着增强,比裸MoS 2 NS的活性高4.3倍。催化活性的这种提高主要归因于DNA / MoS 2 NSs对底物TMB的亲和力增加,从而进一步加速了电子从TMB到H 2 O 2的转移。基于DNA调节的MoS 2开发了NS纳米酶活性,一种比色传感平台,用于以敏感方式轻松检测癌胚抗原(CEA)。有趣的是,通过将适体/ MoS 2 NS系统植入琼脂糖水凝胶中,制造了一种方便,价格合理且无需仪器的便携式测试套件,以通过视觉方式监控CEA 。重要的是,我们的工作阐明了使用DNA增强纳米酶催化的可行性及其在无标记,可移植和可视化的适体靶向生物分子检测中的应用潜力。
更新日期:2020-10-02
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