This study aimed to evaluate the method of fluorescence diagnostics using the developed video-fluorescent equipment with laser excitation of fluorescence and the assessment of the effectiveness of photodynamic therapy via the photobleaching of the chlorin e6. The boundaries of precancerous conditions and cervical neoplasms were established using video-fluorescent diagnostics and then clarified using spectral-fluorescent diagnostics. At a photosensitizer dose of 0.8–1.2 mg kg −1 , fluorescence images of the cervix contrasted well in the pathological tissue compared to the normal surrounding tissue. This made it possible to estimate the photosensitizer distribution in the entire measured area with a resolution of at least 1 mm. The parallel use of spectral-fluorescent diagnostics allows the identification of fluorescence typical for the photosensitizer fluorescence and evaluating its distribution in the studied tissues. The combined use of video- and spectral-fluorescent ...

中文翻译：

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宫颈肿瘤的光谱和视频荧光联合诊断用于光动力治疗

这项研究的目的是使用已开发的视频荧光设备和激光激发荧光来评估荧光诊断方法，并通过二氢卟酚e6的光漂白来评估光动力疗法的有效性。癌前状况和子宫颈肿瘤的界限是使用视频荧光诊断法确定的，然后使用光谱荧光诊断法加以阐明。在光敏剂剂量为0.8–1.2 mg kg -1时，与正常周围组织相比，病理组织中子宫颈的荧光图像对比度很好。这使得有可能以至少1mm的分辨率估算整个测量区域中的光敏剂分布。光谱荧光诊断程序的并行使用可以识别光敏剂荧光的典型荧光并评估其在研究组织中的分布。视频和光谱荧光灯的组合使用...