Heparin-like polymers are promising synthetic materials with biological functionalities, such as anticoagulant ability, growth factor binding to regulate cellular functions, and inflammation mediation, similar to heparin. The biocompatibility of heparin-like polymers with well-defined chemical structures has inspired many researchers to design heparin-like surfaces to explore their biological applications. The concept of the recombination of functional heparin structural units (sulfonate- and glyco-containing units) was proven to be successful in designing heparin-mimicking surfaces. However, besides surface structural units, topographic patterning is also an important contributor to the biological activity of the surfaces modified with heparin-like polymers. In this work, both surface structural units and topographic patterning were taken into account to investigate the vascular cell behaviors on the silicone surfaces. A facile method for the production of patterned bromine-containing polydimethylsiloxane surface (PDMS-Br) was developed from a one-step multicomponent thermocuring procedure and replica molding using a nanohole-arrayed silicon template. Different structural units of heparin-like polymers, i.e. homopolymer of sulfonate-containing sodium 4-vinylbenzenesulfonate (pSS), homopolymer of glyco-containing 2-(methacrylamido)glucopyranose (pMAG), and copolymers of MAG and SS (pSG), were then introduced on the flat and patterned PDMS-Br surface using visible light-induced graft polymerization. For the flat surfaces, compared with the PDMS-Br surface, pSS-grafted and pSG-grafted surfaces significantly increased cell densities of both human umbilical vein endothelial cells (HUVECs) and human umbilical vein smooth muscle cells (HUVSMCs), indicating that they are “vascular cell-friendly”. In contrast, the pMAG-grafted surface showed decreased cell attachment of both HUVECs and HUVSMCs, indicating that the pMAG-grafted surface is “vascular cell-resistant”. Moreover, surface topographic patterning enhanced the cell responses to the corresponding flat surfaces. That is to say, surface patterning can make the “vascular cell-friendly” surface still friendly, and the “vascular cell-resistant” surface much more resistant. The combination of surface structural units and topographic patterning shows promise in the preparation of new heparin-like surfaces with improved cell compatibility that is suitable for blood-compatible biomaterials.

中文翻译：

---

血管细胞对移植有肝素样聚合物的硅树脂表面的反应：表面化学组成与拓扑构图。

类似于肝素的聚合物是具有生物学功能的有前途的合成材料，类似于肝素，其具有抗凝能力，调节细胞功能的生长因子结合和炎症介导作用。具有明确化学结构的类肝素聚合物的生物相容性启发了许多研究人员设计类肝素表面以探索其生物学应用。功能肝素结构单元（含磺酸根和糖基的单元）重组的概念被证明在设计模仿肝素的表面上是成功的。但是，除表面结构单元外，形貌图样也是肝素样聚合物修饰的表面的生物活性的重要贡献者。在这项工作中 同时考虑了表面结构单元和地形图，以研究有机硅表面上的血管细胞行为。从一步式多组分热固化程序和使用纳米孔阵列的硅模板进行仿制成型，开发了一种用于生产带图案的含溴聚二甲基硅氧烷表面（PDMS-Br）的简便方法。肝素样聚合物的不同结构单元，即然后将含磺酸盐的4-乙烯基苯磺酸钠（pSS）的均聚物，含糖的2-（甲基丙烯酰胺基）吡喃葡萄糖（pMAG）的均聚物以及MAG和SS的共聚物（pSG）引入到平坦且有图案的PDMS-Br表面使用可见光诱导的接枝聚合。对于平坦表面，与PDMS-Br表面相比，pSS移植和pSG移植表面显着增加了人脐静脉内皮细胞（HUVEC）和人脐静脉平滑肌细胞（HUVSMC）的细胞密度，表明它们是“血管细胞友好型”。相比之下，pMAG移植的表面显示出HUVEC和HUVSMC的细胞附着均降低，表明pMAG移植的表面具有“抗血管细胞性”。此外，表面形貌图案化增强了细胞对相应平坦表面的响应。也就是说，表面图案化可以使“对血管细胞友好”的表面仍然友好，而对“对血管细胞友好的”表面则更具抵抗力。表面结构单元和地形图图案的结合显示出在制备具有改善的细胞相容性的新肝素样表面方面的希望，该表面适合于血液相容性生物材料。