Abstract Invasive candidiasis is a major challenge to clinical medicine today. However, traditional fungal diagnostic techniques and empirical treatments have shown great limitations. Although efforts are necessarily needed in methodology standardization and multicenter validation, polymerase chain reaction (PCR) is a very promising assay in detecting fungal pathogens. Using a “heat-shock” DNA preparation method, a rapid and simple PCR protocol for quantification of the Candida albicans (C. albicans) ribosomal DNA was established. The PCR assay could detect Candida DNA as low as 10 CFU/mL in samples prepared by the heat-shock protocol, without any cross-reaction with DNA prepared from other Candida spp. and bacterial pathogens. For simulated blood samples, the PCR test sensitivity of whole blood samples was better than that of plasma and blood cells. In the systemic candidiasis murine model, detectable DNA was only observed within 24 h after C. albicans SC5314 injection, which is much shorter than that observed in the kidney.

中文翻译：

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PCR-可检测的念珠菌DNA在系统性念珠菌病鼠模型的血液中存在短时间

摘要 侵袭性念珠菌病是当今临床医学的重大挑战。然而，传统的真菌诊断技术和经验性治疗显示出很大的局限性。尽管在方法标准化和多中心验证方面需要付出努力，但聚合酶链反应 (PCR) 是检测真菌病原体的一种非常有前途的方法。使用“热休克”DNA 制备方法，建立了用于量化白色念珠菌（C. albicans）核糖体 DNA 的快速简单的 PCR 方案。PCR 检测可以检测到通过热休克方案制备的样品中低至 10 CFU/mL 的念珠菌 DNA，而不会与其他念珠菌属制备的 DNA 发生任何交叉反应。和细菌病原体。对于模拟血样，全血样本的PCR检测灵敏度优于血浆和血细胞。在全身念珠菌病小鼠模型中，仅在注射白色念珠菌 SC5314 后 24 小时内观察到可检测的 DNA，这比在肾脏中观察到的要短得多。