Abstract

By using a mixed-ligand approach, a Co(II) coordination polymer (CP) with the chemical formula of [Co(L)(ip)·H₂O]_n (1, L = 1,3-bis(benzimidazol-1-yl)-2-propanol, H₂ip = isophthalic acid) was synthesized under solvothermal conditions. Furthermore, to develop new candidates for cancer treatment, the treatment effect of the compound on the human cervical carcinoma cell line Hela was evaluated. First, the autophagy level of the Hela cell line after compound treatment was measured by flow cytometry. Then, the autophagic genes expression was detected by the reverse transcription-polymerase chain reaction (RT-PCR) determination.

摘要

通过使用混合配体方法，化学式为 [Co(L)(ip)·H ₂ O] _n (1, L = 1,3-bis(benzimidazol- 1-基）-2-丙醇，H ₂ ip = 间苯二甲酸）在溶剂热条件下合成。此外，为了开发新的癌症治疗候选药物，评估了该化合物对人宫颈癌细胞系 Hela 的治疗效果。首先，通过流式细胞术测量化合物处理后 Hela 细胞系的自噬水平。然后，通过逆转录-聚合酶链反应（RT-PCR）测定检测自噬基因的表达。