ABSTRACT

Lead contamination in soil due to anthropogenic activities has amplified and therefore, remediation is of prime significance due to its nonbiodegradability and toxicity effects. This study focuses on lead removal from the soil collected from a rifle range using biosurfactants produced from native microorganisms and edible oils. Native microorganisms in contaminated soil served as a source for biosurfactant production aided by edible vegetable oils such as palm oil and gingelly oil. Preliminary isolation and characterization studies indicated the presence of Pseudomonas aeruginosa that produced biosurfactant and removed lead simultaneously. Batch adsorption experiments showed 96%–99.6% of lead adsorption following Langmuir isotherm model. Lead desorption of 23.6% occurred without biosurfactant. Whereas in the presence of biosurfactants, enhanced desorption of 62.3% was observed. Of both palm oil and gingelly oil derived biosurfactants, the former reached a lead removal efficiency of 93.6% indicating the feasibility and effectiveness of the biosurfactants for contaminated site remediation.

摘要

由于人为活动造成的土壤铅污染已经扩大，因此，由于其不可生物降解性和毒性作用，修复具有重要意义。这项研究的重点是使用从天然微生物和食用油中产生的生物表面活性剂，从步枪系列收集的土壤中去除铅。受污染的土壤中的天然微生物在可食用植物油（例如棕榈油和齿龈油）的辅助下，成为生产生物表面活性剂的来源。初步分离和表征研究表明存在铜绿假单胞菌产生生物表面活性剂并同时去除铅。批量吸附实验表明，按照Langmuir等温线模型，铅吸附的96％–99.6％。在没有生物表面活性剂的情况下发生了23.6％的铅解吸。而在存在生物表面活性剂的情况下，观察到解吸增强了62.3％。在棕榈油和牙龈油衍生的生物表面活性剂中，前者的除铅效率达到93.6％，表明该生物表面活性剂用于污染场地修复的可行性和有效性。